ProductName | HumanChorionicGonadotropin(hCG). |
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SampleType | Serum/Plasma. |
#ofSamples | 1x96well. |
IntendedUse | TheGenWay,Inc.hCGELISAKitisintendedforthequantitativemeasurementofhCGinhumanserumorplasma. |
Principleofthetest | TheGenWayhCGisadirectsolidphasesandwichELISAmethod.ThesamplesanddilutedantihCG-HRPconjugateareaddedtothewellscoatedwithMabtobetasubunit.hCGinthepatient"sserumbindstoanti-hCGMAbonthewellandtheanti-hCGsecondantibodythenbindstohCG.UnboundproteinandHRPconjugatearewashedoffbywashbuffer.Upontheadditionofthesubstrate,theintensityofcolorisproportionaltotheconcentrationofhCGinthesamples.AstandardcurveispreparedrelatingcolorintensitytotheconcentrationofthehCG. |
StorageandStABIlity | 1.Storethekitat2-8°C. 2.Keepmicrowellssealedinadrybagwithdesiccants. 3.Thereagentsarestableuntilexpirationofthekit. 4.Donotexposetestreagentstoheat,sunorstronglight. |
LimitationoftheTest | 1.Thetestresultsobtainedusingthiskitserveonlyasanaidtodiagnosisandshouldbeinterpretedinrelationtothepatient"shistory,physicalfindingsandotherdiagnosticprocedures. 2.Donotusesodiumazideaspreservative.SodiumazideinhibitsHRPenzymeactivities. |