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GenWay/Chagas (Trypanosoma cruzi) IgG – ELISA/GWB-A4E22B/1x96 Assays188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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GenWay/Chagas (Trypanosoma cruzi) IgG – ELISA/GWB-A4E22B/1x96 Assays

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ProductName:Chagas(Trypanosomacruzi)IgG–ELISA

#ofSamples:1x96Assays

IntendedUse:TheChagas(Trypanosomacruzi)IgG-ELISAisintendedforthequalitativedeterminationofIgGclassantibodiesagainstTrypanosomacruziinhumanserumorplasma(citrate).

Introduction:Chagas’diseaseoccursinMiddleandSouthAmerica.Itisaninfectiousdiseasetransmittedtohumansthroughthebitewoundcausedbyaninfectedbugofthefamilyreduviidae.TheinfectioniscausedbyTrypanosomacruziaunicellularparasite.Theinfectionpassesthroughdifferentphasesandcausesanoftenchronicdisease.4to5millionpeopleinSouthAmericaaresufferingfromit.Peoplelivingunderpoorconditionsaremainlyjeopardized.Upto10%ofallinfectionsarefatal;bABIesandneonateshaveaspecialrisk.
Theinfectionistransmittedtohumansbydifferentbugspeciesofthefamilyreduviidae.Theparasitedoesnotenterthehumanbodywiththebitofthebug.Trypanosomacruzilivesinfaecesofthebugandinvadesintohumansbyskinwounds.Newbornsareatriskbyinfectioninutero.AninfectionviabloodtransfusionisalsopossIBLe.
1-2%ofallinfectedpeopleshowsymptomsafteranincubationtimeofonetofourweeks.Childrenupto15yearsoldaremainlyaffected.Thediseasepassesthroughthreedifferentphases.
Duringacutephasefever,diarrhoea,gripes,swollenlymphnodesandswellingofthewholebodyappears.Especiallyinneonatesandinfantsinflammationofheartorbrainarepossible.Theacutephaselastsforca.4weeks.TheacuteChagasdiseaseismostlyadiseaseofchildren.Inmanycasesthediseaseishealedup.
Thefollowinglatencyperiodischaracterizedbylackofsymptomsformostofthepatients.Occasionallyaweakeningoftheimmunesystemtakesplace.Thisphasecanlastseveralyears.
10to20%ofinfectedpeoplereachthechronicphase.Differentinnerorganslikeheart,intestinaltractorneuronalsystemcanbeaffected.Patientsoftendiebesuddencardiacdeathorastheresultofachronicheartinsufficiency.Theprognosisdependsuponthedegreeofheartvariance.
Avarietyofdiagnosticmethodshavebeenused,butdetectionofantibodiestoT.cruziantigensremainsthestrongestmethodtodiagnoseinfection.

Principlesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgG-classantibodiesagainstChagas(Trypanosomacruzi)isbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.
MicrotiterstripwellsareprecoatedwithTrypanosomacruziantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledProteinAconjugateisadded.Thisconjugatebindstoantigen-antibodycomplexes.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.
TheintensityofthisproductisproportionaltotheamountofTrypanosomacruzi-specificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.

StorageandStability:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.

LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasSEROlogicaldata.
Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.
CrossreactionoftheantigenswithantibodiesagainstLeishmania,TrypanosomabruceirhodesienseandTrypanosomabruceigambiensecannotbeexcluded.

ReferencesSOARES,M.B.P.,PONTES-DE-CARAVALHO,L.ANDRIBEIRO-DOS-SANTOS,R.(2001)THEPATHOGENESISOFCHAGAS´DISEASE:WHENAUTOIMMUNEANDPARASITE-SPECIFICIMMUNERESPONSESMEET.AN.ACAD.BRAS.CIENC.73,547-559FERREIRA,M.S.ANDBORGES,A.S.(2002)SOMEASPECTSOFPROTOZOANINFECTIONSINIMMUNOCOMPROMISEDPATIENTS-AREVIEW.MEM.INST.OSWALDOCRUZ,RIODEJANEIRO97,443-457AÑEZ,N.,CRISTANTE,G.ANDROJAS,A.(2004)UPDATEONCHAGASDISEASEINVENEZUELA-AREVIEW.MEM.INST.OSWALDOCRUZ,RIODEJANEIRO99,781-787MACEDO,A.M.,MACHADO,C.R.,OLIVEIRA,R.P.ANDPENA,S.D.J.(2004)TRYPANOSOMACRUZI:GENETICSTRUCTUREOFPOPULATIONSANDRELEVANCEOFGENETICVARIABILITYTOTHEPATHOGENESISOFCHAGASDISEASE.MEM.INST.OSWALDOCRUZ,RIODEJANEIRO99,1-12

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NameChagas(Trypanosomacruzi)IgG–ELISA
RelatedProductNamesChagas(Trypanosomacruzi)IgG–ELISA
StorageThereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.
IntendedUseResearchUseOnly