HMGBiotech/HMGB1 15N labelled for Mass Spectrometry and NMR (50 µg) [HM-180]/50 µg/HM-180

Description

HMGB1 ¹⁵N labelled for Mass Spectrometry and NMR (50 µg) [HM-180]

Full length HMGB1 for Mass Spectrometry is the 25 kDa HMGB1 protein uniformly labelled with isotope ¹⁵N.This protein is suitable for mass spectrometry and NMR studies.This product corresponds to the human sequence and is produced in E.coli using a media in which the only source of nitrogen come from ¹⁵NH₄Cl.The Fully reduced HMGB1 we provide is the natural protein, with no tags or additional amino acids.It has the sequence:

MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE

Molecular Mass: HMGB1 consists of 215 amino acid residues and has a calculated molecular mass of approximately 24.8 kDa. It migrates at a position of approximately 30 kD in SDS-PAGE gels, possibly because of the unusual number of positively charged amino acids it contains.Structure: HMGB1 consists of two fairly rigid, L-shaped DNA-binding domains, each referred to as a ‘HMG box’, and an unstructured tail that ends with 30 consecutive negatively charged amino acids.Purity: The purified protein is >95% homogeneous (electrophoresis and mass spectrometry). It contains no nucleic acids, nor IL-1beta.Buffer: 50 mM HEPES-Na pH 7.9, 500 mM NaCl, 0.5 mM DTT.Storage: the protein is shipped lyophilized. Once resuspended can be stored frozen at -20°C. To avoid cysteine oxidation, DTT 0.5mM is added during protein purification.This product is intended for research only, and cannot be used on humans.

Download HMGB1 for Mass Spectrometry Datasheet

Complete Name: High Mobility Group 1http://hmgbiotech.eu/wp-content/uploads/2017/12/HMGB1-for-mass-spec11.pdfOther Names: Amphoterin-1, High mobility group protein 1,HMG-1, SBP-1, high mobility group protein B1, high-mobility group box 1, Amphoterin, HMG1, HMGB1, SBP-1, recombinant HMGB1, 15N labelled, Mass Spectrometry