产品资料

Nexcelom Bioscience/Cell Fitness Panel Reagents/CSK-V0023-1 25 tests per assay 527/655 nm
470/534 nm

Cell Fitness Panel Reagents

The Cell Fitness Panel Kit is designed for researchers interested in acquiring data on cell sample health and quality.

Many samples in cellular therapy, immunotherapy, and cell line development, are by their nature unique and precious. Patient-derived primary samples are often comprised of a heterogeneous cell population and display a wide range of physiological behaviors. Whether these samples underwent a freeze/thaw cycle, or cell isolation procedure, or have been cultured within a bioreactor for an extended period, the Cell Fitness Panel is used to assess not only the wellbeing of cell samples but also to offer potential insights into samples with low or crashing viabilities.

The Cell Fitness Panel consists of five individual tests to assess viability, vitality, mid and late-stage apoptosis, and reactive oxidative stress.

Ask a Specialist

Shop Online

View All Reagents

ViaStain™ Cell Fitness Panel

To determine cellular health, the kit contains five individual tests:

Viability

More accurate than trypan blue
No red blood cell (RBC) lysis needed
Measure cell viability and concentration of nucleated cells in a single step

Kit provides Acridine orange / Propidium iodide

Vitality

Identify the number of enzymatically active cells vs the number of dead cells in a population
Rapid conversion from non-fluorescing calcein–AM to a brightly fluorescing signal in healthy cells

Kit provides Calcein AM/PI

Mid-stage Apoptosis

Classic apoptosis marker, annexin V/PI, measures percent live, annexin V positive, and necrotic cells
Optimized workflow for rapid staining, imaging, and data analysis

Kit provides Annexin V/PI

Late-stage Apoptosis

Detection of an activated executioner protein Caspase 3/7 is a strong indicator poor cell health
The bright green nuclear fluorescent signal is quickly and easily detected in unhealthy cells

Kit provides Caspase 3

Oxidative Stress

Accumulation of reactive oxygen species (ROS) can result in cell damage and death
The bright green fluorescent signal can be an early indication of an in trouble culture

Kit provides ROS-Total

Run Cell Fitness Panel tests on:

Cellometer Spectrum

Cellaca MX

Description	Catalog Number	Unit Size	Excitation/Emission	Manual	SDS
ViaStain™ Cell Fitness Panel for Cellometer Spectrum	CSK-V0023-1	25 tests per assay	527/655 nm470/534 nm	PDF	US: Full KitEU: Full Kit	Buy Online
ViaStain™ Cell Fitness Panel for Cellaca MX	CSK-V0024-1	1 Cellaca Plate per assay	534/655 nm470/527 nm	PDF	US: Full KitEU: Full Kit	Buy Online

Download the Cell Fitness Panel Assay Templates for Cellometer Spectrum »

Download the Cell Fitness Panel Assay Templates for Cellaca MX »

ViaStain™ Total ROS Green

Oxidative stress is caused by an accumulation of reactive oxygen species (ROS) within cells or tissues. While the production of ROS is part of the normal cellular metabolic processes, the cell’s inability to neutralize these radicals may have a direct impact on cell health, physiological functions, and possibly lead to cell damage or even cell death. Over the years, oxidative stress has been linked with multiple chronic and degenerative diseases such as cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, neurodegenerative diseases, and cancer. The role of antioxidants, including vitamins C and E, polyphenols, and flavonoids, in the reduction of oxidative stress, continues to be a major area of study.

ViaStain™ Total ROS Green kits for Cellometer Spectrum and Cellaca MX uses a unique ROS sensor to quantify ROS in live cells. ROS Green is a cell-permeable stain that generates a strong green fluorescence signal upon reaction with ROS to obtain quantitative cell metrics.

Features and Benefits:

Validated for use on Cellometer Spectrum and Cellaca MX systems
Rapid automatic data analysis using pre-existing templates
Optimized easy-to-follow protocol for best results

Untreated, healthy, Jurkat cells (ROS negative, left) show very little green fluorescence while drug-treated cells (ROS positive, right) produce a bright green ROS positive signal.

Description	Catalog Number	Unit Size	Excitation/Emission	Manual	SDS
ViaStain™ Total ROS Green for Cellometer Spectrum	CSK-V0022-1	300 tests per assay	470/534 nm	PDF	US: Full KitEU: Full Kit	Buy Online
ViaStain™ Total ROS Green for Cellaca MX	CSK-V0019-1	10 Cellaca Plates per assay	470/527 nm	PDF	US: Full KitEU: Full Kit	Buy Online

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Nexcelom Bioscience/Cell Fitness Panel Reagents/CSK-V0023-1 25 tests per assay 527/655 nm 470/534 nm