产品资料

Polyplus Transfection/jetPRIME^®/Polyplus Transfection-163147/1 Ea

HighDNAtransfectionefficiency
Lowamountsofnucleicacid
SuperiorcellviABIlity
ONEreagentforDNAand/orsiRNAtransfections
Cost-effective

Specifications:

Reagent	jetPRIME^®
Moleculedelivered	DNA,siRNA,DNA&siRNA
Applications	Plasmidtransfection Genomeediting Virusproduction siRNAtransfection Co-deliveryofdifferentnucleicacids
Celltypes	Adherentcelllinesgrowninpresenceofserum
Numberoftransfections	1.5mlofjetPRIME^®transfectionreagentissufficienttoperformupto1500transfectionsin24-wellplatesor375transfectionsin6-wellplates
Storage	4°C,stableforatleastoneyearwhenstoredappropriately
Providedwith	jetPRIMEbuffer

Summary:

jetPRIME^®isapowerfulandversatileDNAandsiRNAtransfectionreagentforday-to-dayexperimentsthatleadstoefficientandreliablescientificresults.jetPRIME^®ensureshighDNAtransfectionefficiencyandexcellentgenesilencinginavarietyofadherentcells.jetPRIME^®isalsoidealforDNA/siRNAco-transfectionorco-deliveryofseveralplasmids.FurThermore,ourjetPRIME^®reagentisverygentleoncellssinceitrequireslowamountsofreagentandnucleicacidduringtransfection.

Orderinginformation:

|||114-01##0.1ml##5ml##|||114-07##0.75ml##60ml##|||114-15##1.5ml##2x60ml##|||114-75##5x1.5ml##10x60ml##|||114-75C##5x1.5ml##120ml5Xconc.##|||

Description:

Superiortransfectionefficiency

jetPRIME^®isapowerfultransfectionreagentforday-to-dayexperiments.Itleadstounusuallyhighpercentageoftransfectedadherentcelllinesofvariousoriginsaswellasprimarycells.

Superiortransfectionefficienciesrangingbetween70and90%wereobtainedwithjetPRIME^® reagentversusthetopcompetitor’sreagentforseveralcommonlyusedcelllines(Fig.1).

jetPRIME - Efficiency in different cell lines — **Fig.1:ComparativetransfectionefficiencyofjetPRIMEversusitsmaincompetitor.**TransfectionefficiencywasassessedbyFACSanalysisinvariouscelllines24haftertransfectionin24-wellplates.Conditionsusedwereaccordingtothemanufacturer’srecommendationforL2K(0.8-1µgplasmidDNA+1.6-2µlreagentperwell)andforjetPRIME^®(0.5µgplasmidDNA+1µlreagentperwell).

Cost-effective:lessDNAandlessreagent

jetPRIME^® isapowerfulinvitrotransfectionreagentthatrequiresasmallamountofreagentandplasmidDNA,makingitsuseverycost-effective (Table1).

jetPRIME - table competitors — **Table1:Recommendedconditionstouse.**AmountsofDNAandreagent(jetPRIME^®andcompetitors)perwellin6-wellplatefortransfectionaccordingtomanufacturers’recommendations.

Inadditiontoreducingcosts,usinglessDNAalsominimizesadversecytotoxiceffectstriggeredbytransfection.Hence,jetPRIME^® isthereagentofchoiceforhightransfectionefficiencywithexcellentcellviability.

Bettercellviability

jetPRIME^® isextremelygentleoncellsduringtransfectionleADIngtoincreasedcellviabilityandimprovedtransfectionresults.CellstransfectedwithjetPRIME^® arehealthy,whilemajorcytotoxicityisobservedwithcompetitors(Fig.2).

jetPRIME - transfected cells — **Fig.2:Cellviability24haftertransfection.**PhasecontrastmicroscopyofHeLacells24haftertransfectionsperformedaccordingtothemanufacturer’srecommendationsforeachreagent.

Easytouseprotocol

jetPRIME^® isaneasy-to-usetransfectionreagent:

Fastandeasytoscaleupanddown
CompatIBLewithserumandantibiotics

jetPRIME - protocol — **Fig.3:jetPRIME^®protocol.**ConvenientprotocolforDNA,siRNAandco-transfectionofDNAandsiRNA.

Takealookatour ExpertTips inGeneticEngineeringandBiotechnologyNews.

VIDEO:DNAtransfectionusingjetPRIME

Polyplus-transfectionandGreinerBio-Onehavepartneredtoprovideournewesteducationalwebinar, « ADifferentApproachtoAchieveaHigherTransfectionEfficiency ». Hearthefullseminarrecording.

Applications:

Plasmidtransfection

jetPRIME^®leadstoremarkablyhighpercentagesoftransfectedadherentcelllinesofvariousorigin,aswellasprimarycells(Table1).

jetPRIME - cell list — **Table1:TransfectionefficiencyofvariouscelltypesusingjetPRIME^®.**ThepercentageofGFP-positivecellswasdeterminedbyFACSanalysis24haftertransfection.

PlasmidsaresmallcircularDNAmoleculesthatarecommonlyfoundinbacteria.PlasmidsexistandreplicateseparatelyfromchromosomalDNAandinbacteriatheyoftencarrygenesthatarebeneficialforbacterialsurvival.Plasmidscanbedeliberatelyintroducedintodesiredcellsandutilizedtooverexpressageneofinterestinaspecificcellline.ThisprocedureiscalledDNAtransfectionandisacommonlyusedmethodforstudyinggenefunctionorproteinofinterest.

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Genomeediting

TheuseoftheCRISPR/Cas9systeminmammaliancellshasrecentlyemergedasaveryconvenientwaytomodifythecellgenomeataspecificlocus.Itinvolvestransienttransfectionintomammaliancellsofeither(a)oneorseveralplasmidscodingforCas9,thespecificgRNAandeventuallythesequencetobeinserted,or(b)amixofoneortwoplasmidsandanRNAmolecule(thegRNA).

Availableapplicationnote: CRISPR/Cas9-mediatedgenedisruptionusingjetPRIME^®.

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Virusproduction

jetPRIME^® transfectionreagentishighlyeffectiveforroutinevirusproductionofbothAAVandlentivirusinadherentcellsgrowninclassicalmediasuchasDMEMinpresenceofserum.

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siRNAtransfection

jetPRIME^® leadstoover90%knockdownofendogenousgeneexpressioninavarietyofcelllines.Forexample,jetPRIME^®-mediatedtransfectionofHeLacellswith10nMsiRNAduplexestargetingendogenouslaminA/CinHeLacellsdrasticallyreduceslaminA/Cgeneexpressiontobarelydetectablelevel(Fig.1).

jetPRIME - siRNA — **Fig.1:EndogenouslaminA/CsilencingusingjetPRIME^®.**HeLacellsweretransfectedwith10nMof21-merlaminA/CsiRNA.After48h,laminA/CsilencingwasassessedbyimmunofluorescencemicroscopyusinganantibodyagainstlaminA/C.

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Co-transfectionofdifferentnucleicacids

jetPRIME^® iswellsuitedforDNAandsiRNA/miRNAco-transfectionexperimentsorco-deliveryofseveralDNAplasmids.

WeperformedDNAandsiRNAdeliverywithjetPRIME^®andobservedhighlyefficientgenesilencinginavarietyofcelllineswithverylowtoxicity.Over90%silencingisachievedinadherentcells,using10nMsiRNA(Fig.2).

jetPRIME - cotransfection silencing — **Fig.2:ExogenousluciferasesilencinginseveralcelllinesafterDNA&siRNAcotransfectionusingjetPRIME^®**.Experimentswereperformedwith400ngp4CMV-Lucand10nMofluciferasesiRNAperwellin6-wellplates.

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Citations:

HereisaselectionofrelevantreferencesusingjetPRIME®,moreareavailableinourPolyplus-transfectionDatabase.Charrier,C.,Joshi,K.,Coutinho-Budd,J.,Kim,J.E.,Lambert,N.,deMarchena,J.,Jin,W.L.,Vanderhaeghen,P.,Ghosh,A.,Sassa,T.,Polleux,F.(2012).InhibitionofSRGAP2functionbyitshuman-specificparalogsinducesneotenyduringspinematuration.,Cell 149,923-3.Ichim,G.,Genevois,A.L.,Menard,M.,Yu,L.Y.,Coelho-Aguiar,J.M.,Llambi,F.,Jarrosson-Wuilleme,L.,Lefebvre,J.,Tulasne,D.,Dupin,E.,LeDouarin,N.,Arumae,U.,Tauszig-Delamasure,S.,Mehlen,P.(2013).TheDependenceReceptorTrkCTriggersMitochondria-DependentApoptosisuponCobra-1Recruitment.,MolCell 51,632-4.Rodriguez,M.I.,Peralta-Leal,A.,O’Valle,F.,Rodriguez-Vargas,J.M.,Gonzalez-Flores,A.,Majuelos-Melguizo,J.,Lopez,L.,Serrano,S.,deHerreros,A.G.,Rodriguez-Manzaneque,J.C.,Fernandez,R.,DelMoral,R.G.,deAlmodovar,J.M.,Oliver,F.J.(2013).PARP-1regulatesmetastaticmelanomathroughmodulationofvimentin-inducedmalignanttransformation.,PLoSGenet 9,e1003.Shi,J.,Zhao,Y.,Wang,Y.,Gao,W.,Ding,J.,Li,P.,Hu,L.,Shao,F.(2014).InflammatorycaspasesareinnateimmunereceptorsforintracellularLPS.,Nature 514,187-9.Thomas,M.G.,Luchelli,L.,Pascual,M.,Gottifredi,V.,Boccaccio,G.L.(2012). Amonoclonalantibodyagainstp53cross-reactswithprocessingbodies.,PLoSONE 7,e3644.

Quality:

EverybatchofjetPRIME^®reagentistestedin-housebyDNAtransfectionofHeLacellswithaGFP-expressingplasmidandeachvialofreagentisprovidedwithCertificateofAnalysis.

Testimonials:

“jetPRIMEgavebettertransfectionefficienciesthanotherreagentstestedandmuchlesscelldeathwasnoticed. Thecellsdon’tseemtomindbeingtreatedwiththetransfectionreagent,thereisnoneedtochangethemediaanyway. I’vealreadyorderedabiggervolumeofjetPRIMEandhaveusedhalfofitalready!Willpossiblybeorderingsomemoresoon!”

KimberleyS,RoyalVeterinaryCollege,UK

“IalreadyorderedjetPRIME.Itwasveryefficientandeasytouse!Alsothecellswereinagoodstateaftertransfection.Goodproduct!”

CosimaR,UniversityHospitalErlangen,DE

“Verygoodtransfectionefficiency,bettervalueandeaseofuse”

SeanH.MDAndersonCancerCenter,USA

“jetPRIMEhaslowtoxicityandwithlessamountofDNAandthetransfectionreagentcomparableexpressioncanbeachieved.IwouldpreferusingjetPRIMEoverotherreagentsfortheabovementionedreasons.”

VijayalakshmiA,BaylorCollegeofMedicine(BCM),USA

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Polyplus Transfection/jetPRIME®/Polyplus Transfection-163147/1 Ea

Specifications:

Summary:

Orderinginformation:

Description:

Superiortransfectionefficiency

Cost-effective:lessDNAandlessreagent

Bettercellviability

Easytouseprotocol

VIDEO:DNAtransfectionusingjetPRIME

Applications:

Plasmidtransfection

Genomeediting

Virusproduction

siRNAtransfection

Co-transfectionofdifferentnucleicacids

Citations:

Quality:

Testimonials:

Polyplus Transfection/jetPRIME^®/Polyplus Transfection-163147/1 Ea