产品资料

Polyplus Transfection/jetMESSENGER™/Polyplus Transfection-163148/1 Ea

Unmatchedtransfectionefficiency
Highefficiencyonawidevarietyofdifficulttotransfectcells
OutperformDNAtransfectionbyswitchingtomRNA
Extremelygentleoncells
Noriskofgenomeintegration
PerfectlysuitedforCRISPR/Cas9geneediting,iPSgeneration,stemcelldifferentiationandimmunotherapyassays

Specifications:

Reagent	jetMESSENGER™
Moleculedelivered	mRNA
Applications	Geneexpression GenomeEditing Stemcelldifferentiation Reprogramming Immunotherapy
Celltypes	Hardtotransfectcells AdherentandsUSPensioncells Primarycells(Neurons,Stemcells,Immunecells,fibroblasts,…) Post-mitoticcells
Numberoftransfections	1.5mlofjetMESSENGER™transfectionreagentissufficienttoperformupto1500transfectionsin24-wellplatesor375transfectionsin6-wellplates
Storage	4°C,stableforatleastoneyearwhenstoredappropriately
Providedwith	mRNAbuffer

Summary:

TakeyourgeneexpressiontothenextlevelbyswitchingtomRNA!

Orderinginformation:

|||150-01##0.1ml##10ml##|||150-07##0.75ml##60ml##|||150-15##1.5ml##2x60ml##|||

Description:

OutperformsDNAtransfectioneverytime

jetMESSENGER™isahighlyefficientandgentlemRNAtransfectionreagent.mRNAtransfectionpresentsmanyadvantagesversusDNAtransfection:

noriskofgenomeintegration,hencenogenomemodificationofthetransfectedcell
nopromoterregulationissue
noneedtoreachthenucleusforefficientexpression
moregentleprocess

jetMESSENGER™hasbeenspecificallydesignedtoofferoutstandingtransfectionefficiencyincellsthatareusuallyhardtotransfect,finallyallowingrelevantgeneexpressionexperimentsinalmostallcelltypes(Fig.1).

jetMESSENGER outperforms DNA competitor

Fig.1.jetMESSENGER™outperformsitsmainDNAtransfectionreagentcompetitor.TransfectionefficiencywasassessedbyFACSanalysisinvariouscelllines24haftertransfectionofeGFPmRNA(5meC,pseudo-uridine,TriLink™)orplasmidDNAencodingforeGFP.Conditionswereused accordingtothemanufacturers’recommendation.

Worksonavarietyofcelllines

WithjetMESSENGER™,achieveoutstandingtransfectionefficiencyinawidevarietyofcelllines,fromadherentandsuspensioncancercells,tosensitiveprimarycellsandneurons.jetMESSENGER™isalsoperfectlysuitableforreprogrammingexperimentsinfibroblastsandstemcells(Table1).

jetMESSENGER - Table transfection efficiency — **Table1.jetMESSENGER™leadstohightransfectionefficiencyinawidevarietyofcelltypes.**

Easytouse

mRNAtransfectionisaseasyasDNAtransfection:justdilutethemRNAintheprovidedbuffer,addjetMESSENGER™tothedilutedmRNAandaddthemixtothe cells’growthmediuminpresence/absenceofserum(compatIBLewithantibiotics) (Fig.2).

jetMESSENGER - Protocol — **Fig.2.Easytransfectionprocess**

Extremelygentletocells

Byeliminatingtheneedtoreachthenucleusforefficientexpression,jetMESSENGER™allowstransfectionofquiescentandslowdividingcells.Inaddition,jetMESSENGER™operatesthroughanextremelygentleprocess.CellviABIlityremainsextremelyhighduringtransfectionandcellmorphologyismaintained(Fig.3).Makeyourtransfectionexperimentsphysiologicallyrelevant!

jetMESSENGER - Microscopy

Fig.3.jetMESSENGER™providesabettercellviabilityandahigherproteinexpressionthanDNAtransfection.PrimaryRatCorticalneurons,HepG2andmousestemcellswereanalyzed48haftertransfectionusingphasecontrastandfluorescentmicroscopy.ThetransfectionswereperformedwithjetMESSENGER™/eGFPmRNA(5meC,pseudo-uridine,Trilink™)andL2K/eGFPplasmidDNAaccordingtothemanufacturers’recommendationsforeachreagent.

Applications:

mRNA transfection

jetMESSENGER™isperfectlysuitedforgeneexpressionindifficulttotransfectcellssuchasneurons,primarycells, stemcellsandvariouscancercelllines,throughmRNAtransfection(Fig.1&Fig.2).

jetMESSENGER - Cells

Fig.1.jetMESSENGER™-mediatedtransfectionindifferenthardtotransfectcells.Variouscelllineswereanalyzedbyfluorescentmicroscopy48haftertransfection.TransfectionswereperformedwithjetMESSENGER™/eGFPmRNA(5meC,pseudo-uridine,Trilink™)accordingtothemanufacturer’srecommendation.DataonOVCAR-3,NCCIT,MDA-MB-231&SW1353celllinesarekindlyprovidedbyInsermU1199«BioTICLA»(Caen,France).

jetMESSENGER - Primary mouse lung endothelial cells

Fig.2.jetMESSENGER™issuitabletotransfectmRNAsinprimarymouselungengothelialcells. Primarylungcellswereanalyzedbyfluorescentmicroscopy48hoursposttransfection.CotransfectionwasperformedwithmodifiedRNAsfornuclearGFPandmCherryusingjetMESSENGER™.DataarekindlyprovidedbyT.Itkin, WeillCornellMedicine,USA.

Neurologyapplications

jetMESSENGER™leadstohightransfectionefficiencyinprimaryneurons,whilemaintainingaphysiologicalandhealthycellmorphologyforseveraldays,allowinglongtermexpressionforneurologyapplications(Fig.3.).

Fig.3.jetMESSENGER™issuitabletotransfectmRNAinprimaryratcorticalneurons.Primaryratcorticalneuronswereanalyzedbybright-fieldandfluorescentmicroscopy48haftertransfection.TransfectionwasperformedwithjetMESSENGER™/eGFPmRNA(5meC,pseudo-uridine,Trilink™)accordingtothemanufacturer’srecommendations.

CRISPR/Cas9applications

jetMESSENGER™isperfectlysuitedforRNA-basedgenomeeditingusingCRISPR/Cas9 .

Cellreprogramming

CellstransfectedwithjetMESSENGER™showahighviabilityandalongtermexpressionoftheproteinofinterest,makingjetMESSENGER™theidealtransfectionreagentforreprogrammingstudies.Itisnowpossibletoturnanycellofthebodyintopluripotentstemcells.Forexample,itispossibletousemRNAtransfectionwithjetMESSENGER™totransformfibroblastsintoIPs.

Fig.4.jetMESSENGER™issuitabletotransfectmRNAinBJcells(humanfibroblast).BJcellswereanalyzedbybright-fieldandfluorescentmicroscopy48haftertransfection.TransfectionwasperformedwithjetMESSENGER™/eGFPmRNA(5meC,pseudo-uridine,Trilink™)accordingtothemanufacturer’srecommendations.

Immunotherapyapplications

EfficientcancerandimmunecellstransfectionwithjetMESSENGER™offersawiderangeofcancerandimmunotherapyapplications.

Citations:

“ApplicationofjetMESSENGERreagenttointroducemodifiedRNAmoleculesintoprimarylungmouseendothelialcells(acellpopulationwhichisgenerallyhardtotransfectwithDNA/RNAusingnon-viralmethodology)resultedwiththehighesttransfectionrate(>70%)andwiththelowesttoxicityeffectsascomparedtoothertestedRNA/DNAtransfectionreagents(jetPEI,TransIT,&RNAiMAX).”ItkinT.,WeillCornellMedicine,NewYork,UnitedStates“jetMESSENGERappearsasaveryeffectivereagentforRNAtransfectioninhumancancercellsofvariousorigins.TheexpressionlevelintensityandthetransfectedcellsproportionwereveryimpressiveascomparedtoconventionalDNAtransfectionreagents,withoutanysignofcytotoxicity.”BrotinE.,AbeilardE.,DenoyelleC.,PoulainL.InsermU1199«BioTICLA»,Caen,France“TheprotocolandhandlingofjetMESSENGERKitwasverysimpleandeasytouseincomparisontoothermRNAkitswetested.Inourhands,jetMESSENGERistheonlycomponentthatresultedin60%transfectionefficiencyinperitonealmacrophages,inlessthan9hwhilenotaffectingcellviability.”HerbM.,UniversityofCologne,Germany

Quality:

EverybatchofjetMESSENGER™mRNAtransfectionreagentistestedin-housebymRNAtransfectionofCaCo-2cellswithaGFP-expressingmRNAandeachvialofreagentisprovidedwithaCertificateofAnalysis.

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