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Advanced BioMatrix/Lifeink^® 200//5278-5ML

ProductDescription

ForprintingwithLifeink^®200,wehighlyrecommendusingtheFRESHprintingtechniqueandprintingat2-8°C.Threedimensional(3D)gelsallowforthestudyoftheeffectsofthemechanicalpropertiesoftheextracellularmatrix(ECM),suchasdensityandrigidity,oncelldevelopment,migration,andmorphology.Unlike2Dsystems,3Denvironmentsallowcellextensionstosimultaneouslyinteractwithintegrinsonallcellsurfaces,resultingintheactivationofspecificsignalingpathways.Gelstiffnessorrigidityalsoaffectscellmigrationdifferentlyin3Dversus2Denvironments.

Abioinkthatresembleslifeshouldincludethefollowingtraits:HighPrintResolution,shear-thinning,strongmechanicalstrength,superiorcytocompatibility,allowforcellularremodeling,andisbiomimetic.Lifeink^®200fulfillsthoserequirements.

AdvancedBiomatrixoffersLifeink^®200,abioinkthatisa35mg/mltypeIcollagen.Lifeink^®200ispHneutralcollagenwithphysiologicalsaltconcentration.Thecollagenstartingmaterialispurifiedusingacontrolledmanufacturingprocess.

ReadthegroundbreakingpublicationusingLifeink^®200forbioprintingheartcomponentshere.

Lifeink^®200bioinkhasthefollowingcharacteristicsasshownbelow:

Parameter,Testing,andMethod	Lifeink^®200#5278
SterilizationMethod	Filtration,AsepticProcessing
Sterility-USPmodified	Nogrowth
Form	OpaqueGel/Paste
PackageSize	5mL
StorageTemperature	2-8°C
PrintTemperature	2-8°C
ShelfLife	Minimumof6monthsfromdateofreceipt
Endotoxin-LAL	<10.0EU/mL
pH	6.9-7.6
Osmolality(mOsmoH2O/kg)	290-370
SourceMaterial	BovineCollagen
ElectrophoreticPattern-CoomassieBlue	Characteristic
CollagenConcentration-Biuret	30-45mg/mL
CollagenPurity-SilverStaining	>99%
ContinuousFlowExtrusion	Pass

DirectionsforUse

DownloadthefullPDFversionorcontinuereADIngbelow:

Note:Employasepticpracticestomaintainthesterilityoftheproductthroughoutthepreparationandhandlingofthecollagenandothersolutions.

Note:Itisrecommendedthatthecollagenandotherworkingsolutionsbechilledduringthepreparationofthecollagen.

Note:Vortexingofcollagenisnotrecommendedatanystep.

Note:EnsurethatNObubblesenterthesystem.Bubblesinthesystemwhilemixingwillturnyourinkintoafoam-likematerial.

Note:WerecommendprintingLifeink^®at2-8°Cintoagelatinslurrysupport(FRESHmethod)forbestresults.

CellAdditiontoBioink:Note:Keepcollagenchilledthroughouttheseprocesses

Addtheconcentratedcellsuspensionincellculturemediatoasterilesyringe.

Videoforstep1

Note:Werecommendafinalbioinkcellconcentrationof5X10⁶/mLorgreater.

Note:Forbestresults,add2mLofcellsuspensionper5mLofcollagenBioink.Useasimilarratioforsmallervolumes.

Placesterilecouplerontheendofthesyringewiththecellsuspension.
Slowlypushplungerinuntilmediaformsaslightexternalmeniscusabovetheendofthecoupleronthesyringe.
Removecapfromthesyringewithcollagenandslowlypushplungerinuntilcollagenformsaslightexternalmeniscusabovetheendofthesyringe.
Couplethesyringewithcellstothesyringewithcollagen.(Ensurethattherearenoairbubblesinthesystem.The“externalmeniscus”onbothsyringeshelpsensurethattherearenoairbubblesintroduced).
Slowlypushplungersbackandforth~40timestoensurethoroughmixing.Endwithallofthematerialinthesyringetobeusedforprinting.

Videoforsteps2-6

Thecell-ladenbioinkisnowreadyforpositivedisplacementprinters.Allow5-10minutesforthesyringetoequilibratebackto2-8°Cbeforeprintingforbestresults.

Note:Forpneumaticprinters,transferthecollagenintoanappropriatesyringeusingthecoupler.Thenewsyringeshouldhavethesealinserted,buttheplungerremoved.Centrifugethesyringeat2000RPMfor1minuteaftertransferringthecollagentoremoveanyairbubbles.Donotdothecentrifugestepiftherearecellsalreadymixedin.

Watchthisvideoforhelp

GeneralPrintingNotes:

Touseasmallervolumeofcollagen,simplytransferthedesiredamountofcollagentoanothersyringe,usingtheprovidedsterilecoupler.Toremovetheairfromthenewsyringe,youcandoeitherofthefollowing:
Centrifugethesyringe(capped)withthecappointinguptocausetheairtoaccumulateatthecap.Evacuatetheair.
Centrifugethesyringe(capped)withthecappointingdown,andthenuseahemostattosqueezethesyringewhilepushingtheplungertoallowtheairtoescape.

Removingairwithahemostatvideo

Completeyourprintwithin1hourofcelladditiontosyringe/bioinkforbestcellviABIlityresults.
WhenprintingwithFRESHgelatinslurry,allowthefinalprintedstructuretoincubateat37°Cfor30to60minutesandthenreplacethegelatinwithmedia.
Avoidbubbles.

ProductCellAssay

FibroblastsDay2

AdiposeDerivedStemCellsDay1

AdiposeDerivedStemCellsDay6

Fibroblastsday1

Fibroblastsday5

ProductBioprints

FullScaleHumanNeonatalHeart

HeartValve-Closed

HeartValve-Open

Meniscus

SimpleScaffold

Justahand...

(Canyouseethesinglelayerthinfingernails?)

ProductReferences

ReferencesforLifeink^®collagenbioinks:

Lee,A.etal.3Dbioprintingofcollagentorebuildcomponentsofthehumanheart.Science365,482–487(2019).

Maxson,EvaL.,etal."Invivoremodelingofa3D-Bioprintedtissueengineeredheartvalvescaffold."Bioprinting(2019):e00059.

Filardo,G.etal.Patient-specificmeniscusprototypebasedon3Dbioprintingofhumancell-ladenscaffold.Bone&JointResearch8,101–106(2019).

Schmitt,T.AnalysisandClassificationof3-DPrintedCollagen-BioglassMatricesforCellularGrowthUtilizingArtificialNeuralNetworks.UniversityThesis(2018).

Balakhovsky,Y.M.,Ostrovskiy,A.Y.&Khesuani,Y.D.EmergingBusinessModelsTowardCommercializationofBioprintingTechnology.3DPrintingandBiofabrication1–22(2017).doi:10.1007/978-3-319-40498-1_25-1

Fox,S.etal.Asimplifiedfabricationtechniqueforcellularizedhigh-collagendermalequivalents.BiomedicalMaterials14,041001(2019).

ProductCertificateofAnalysis

Noresultfor.

SafetyandDocumentation

SafetyDataSheet

CertificateofOrigin

DeclarationofMaterialSource

ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

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Advanced BioMatrix/Lifeink® 200//5278-5ML