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Lifeink® 240 is an acidic Type I collagen bioink at a concentration of 35 mg/ml for extrusion-based 3D bioprinting. The product is a highly concentrated acidified collagen solution intended to be extrusion printed employing FRESH bioprinting (see LifeSupport® Catalog No. 5244-8GM).
Lifeink® 240 produces printed structures with a high print resolution and good mechanical strength. The product is formulated in an acidic saline buffer solution. Once the collagen is printed into LifeSupport®, the pH and salts concentration of the printed structure become physiological. Cells can then be seeded onto the printed structure allowing for cell adherence and cellular remodeling of the 3D bioprinted structure.
Parameter, Testing, and Method | Lifeink®240 #5267 |
Sterilization Method | Filtration, Aseptic Processing |
Sterility - USP modified | No growth |
Form | Clear Viscous Solution |
Package Size | 5 mL |
Storage Temperature | 2-8°C |
Print Temperature | 2-25°C |
Shelf Life | Minimum of 6 months from date of receipt |
Endotoxin - LAL | <10.0 EU/mL |
pH | 3.0-5.0 |
Osmolality (mOsmo H2O/kg) | 450-700 |
Source Material | Bovine Collagen |
Electrophoretic Pattern - Coomassie Blue | Characteristic |
Collagen Concentration - Biuret | 30-40 mg/mL |
Collagen Purity - Silver Staining | >99% |
Continuous Flow Extrusion | Pass |
Download the full PDF version or continue reading below:
Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation and handling of the collagen and other solutions.
Note: Ensure that NO bubbles enter the system if mixing other materials. Bubbles in the system while mixing will turn your ink into a foam-like material.
Note: Cells should not be added directly to the Lifeink® 240 bioink since it is an acidic formulation.
Addition of other components to Lifeink®240 Bioink:
Note: Components other than cells can be added to the Lifeink® 240 bioink before bioprinting as long as these components are compatible with acidic pH conditions.
Video for step 1
Note: The video shows cells being added to the bioink. Other materials can be added to the Lifeink® 240 bioink in lieu of cells. Cells should NOT be added directly to this bioink. For cellular bioprinting, use Lifeink® 200 #5278-5ML.
Note: For best results, add no more than 5 mL of other components per 5 mL of collagen bioink. Use a similar ratio for smaller volumes.
Video for steps 2-6
Note: For pneumatic printers, transfer the collagen into an appropriate syringe using the coupler. The new syringe should have the seal inserted, but the plunger removed. Centrifuge the syringe at 2000 RPM for 1 minute after transferring the collagen to remove any air bubbles.
Watch this video for help
General Printing Notes:
Removing air with a hemostat video
For more directions on FRESH printing, please visit our LifeSupport® Directions for Use.
References for Lifeink®collagen bioinks:
Lee, A.et al.3D bioprinting of collagen to rebuild components of the human heart.Science365,482–487 (2019).
Maxson, Eva L., et al. "In vivo remodeling of a 3D-Bioprinted tissue engineered heart valve scaffold."Bioprinting(2019): e00059.
Filardo, G.et al.Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold.Bone & Joint Research8,101–106 (2019).
Schmitt, T. Analysis and Classification of 3-D Printed Collagen-Bioglass Matrices for Cellular Growth Utilizing Artificial Neural Networks.University Thesis (2018).
Balakhovsky, Y. M., Ostrovskiy, A. Y. & Khesuani, Y. D. Emerging Business Models Toward Commercialization of Bioprinting Technology.3D Printing and Biofabrication1–22 (2017). doi:10.1007/978-3-319-40498-1_25-1
Fox, S.et al.A simplified fabrication technique for cellularized high-collagen dermal equivalents.Biomedical Materials14,041001 (2019).
Safety Data Sheet
Certificate of Origin
Declaration of Material Source
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
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