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assaybiotechnology/Cyclin B1 (Phospho-Ser126) Antibody/A0068/100ul

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Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures

ProductNameCyclinB1(Phospho-Ser126)Antibody
SynonymsCCN-2;CCNB;CCNB1;CCNB1-RS1;CYCB;CYCB1;G2/mitotic-specificcyclinB1
ImmunogenTheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCyclinB1aroundthephosphorylationsiteofSer126.
ImmunogenRange91-140
ClonalityPolyclonal
ApplicationDilutionsWB:1:500~1:1000IHC:1:50~1:100ELISA:1:5000
SpecificityCyclinB1(Phospho-Ser126)AntibodydetectsendogenouslevelsofCyclinB1onlywhenphosphorylatedatSer126.
PurificationTheantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide.
PhysicalFormRabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.
Storage/StABIlityStableat-20°Cforatleast1year.
NCBIGeneSymbolCCNB1
DatabaseLinks

GeneID: 891 Swiss-Prot#: P14635

OMIM#: 123836 Unigene#: Hs.23960

TargetModificationPhospho
ModificationSitesH:S126M:S123R:S116
ApplicationImages
Image1Immunohistochemistryanalysisofparaffin-embeddedhumanlungcarcinoma,usingCyclinB1(Phospho-Ser126)Antibody.Thepictureontherightisblockedwiththephosphopeptide.
Image2WesternblotanalysisoflysatesfromNIH/3T3cellstreatedwithEGF200ng/ml15",usingCyclinB1(Phospho-Ser126)Antibody.Thelaneontherightisblockedwiththephosphopeptide.
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