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eEnzyme/Recombinant Enterokinase (rEK)/EK-001P/1 Ea

EK-001P

Name

RecombinantEnterokinase(rEK)

Description

Recombinant   Enterokinase   (rEK)   is   the   catalytic   subunit   of  bovineenterokinase,whichisexpressedbytheyeastPichiapastorisandpurifiedtoyieldahighenzymeactivitypreparation.rEKrecognizesthesequenceAsp-Asp-Asp-Asp-Lysandcleavesthepeptidebondafterthelysineresidue.Theenzymecanbeusedtocleaveanyfusionproteinthatcarriesthissequence.

Source

Yeast

UnitDefinition

One unit of rEK is the amount of enzyme that will cleave 20µg ofthioredoxin-chloramphenicciacetyltransferasefusionproteincontaininganenterokinasecleavagesite(Asp-Asp-Asp-Asp-Lys)to90%completionat37℃in16hours

Size

1000units(1U/µl)

 

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