ChickenAorta TotalRNAisextractedfromfreshlyharvestedtissuesofsinglehealthynormalanimaldonorusingclassicalguanidineisothiocyanate–phenol:chloroformextractionmethodwhichallowstherapidisolationoftotalRNAincludingmicroRNAs. RNA istreatedwithRNase-freeDNase-1toremoveresidualDNA,preciselyquantified,andstoredat-80oC.
QualityControl: TheintegrityofeachRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1). ResidualDNAcontaminationistestedbyPCR.
Applications: RNAisidealforNorthernblotting,ribonucleaseprotectionassay,SInucleaseassay,RT-PCR/Q-PCR/RACEanalysis,CDNAsynthesis,RNAdifferentialdisplay,microRNAstudies,andpurificationofmRNAforlibraryconstruction.
Packing/shipping:TotalRNAsampleisroutinelyprovidedinRNase-freewaterataconcentrationof1mg/mlandshippedondryice.Samplecanalsobeprovidedin1mMsodiumcitrate,0.1mMEDTA,oranyotherRNAstoragebuffer.
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn