Mouse mammary gland D7 involution total protein (protein lysate) is isolated from freshly harvested mammary gland. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of 7 mammalian protease inhibitors to minimize proteolysis, precisely quantified, and stored at -80oC.
Quality Control: Quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE using Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.
Applications: Total protein is ready for immediate use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, protein-protein interaction, and tissue specific expression.
Packing/shipping: The extracted protein is provided in vials at a concentration of 5mg/ml and shipped on dry ice. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at zinfo@zyagen.com
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn