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Agrisera/CP43 | IsiA homolog of plant CP43 positive control/quantitation standard/AS10 111S/188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Agrisera/CP43 | IsiA homolog of plant CP43 positive control/quantitation standard/AS10 111S/
productinformation
Background

Thisproductisarecombinantproteinstandard,source:SynechocystisstrainPCC6803.

Host
Clonality
Clone
Purity
FormatLyophilized
Quantity250µl
ReconstitutionForreconstitutionadd225µlofmilliQwater
Storage

storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.

Testedapplicationswesternblot(WB)
Relatedproducts

AS06111|anti-CP43|IsiAhomologofplantCP43antibodies

Collectionofglobalantibodies

Collectionofantibodiestophotosyntheticproteins

Plantandalgalproteinextractionbuffer

Additionalinformation

TheIsiAproteinstandardcanbeusedincombinationwithanti-IsiAantibodiestoquantitateIsiAfromarangeofcyanobacteria.GlobalantibodiesareraisedagainsthighlyconservedaminoacidsequencesintheIsiAprotein.

Quantitativewesternblot: detailedmethoddescription,videotutorial

applicationinformation
Recommendeddilution

Standardcurve:3loadsarerecommended(2.5and10μl).
Formostapplicationsasampleloadof0.2μgofchlorophyllwillgiveaIsiAsignalinthisrange.

Positivecontrol:a2μlloadperwellisoptimalformostchemiluminescentdetectionsystems.

ThisstandardisstABIlizedandreadyanddoesnotrequireheatingbeforeloADIngonthegel.Pleasenotethatthisproductcontains10%glycerolandmightappearasliquidbutisprovidedlyophilized.Allowtheproductseveralminutestosolubilizeafteraddingwater.MixthoroughlybutgentlyTakeextracaretomixthoroughlybeforeeachuse,astheproteinstendtosettlewiththemoredenselayerafterfreezing.

Expected|apparentMW

27kDa(slightlylargerthannativeproteinduetoHis-tag)

Confirmedreactivity
Predictedreactivity
Notreactivein
Additionalinformation

Concentration:afteradding225 µlofmilliQwaterfinalconcentrationofthestandardis0.15pmoles/µl

Proteinstandardbuffercomposition:Glycerol10%,TrisBase141mM,TrisHCl106mM,LDS2%,EDTA0.51mM,SERVA®BlueG2500.22mM,PhenolRed0.175mM,pH8.5,0.1mg/mlPefaBlocproteaseinhibitor(Roche),50mMDTT.

Thisstandardisready-to-loadanddoesnotrequireanyadditionsorheating.Itneedstobefullythawedandthoroughlymixedpriortousing.Avoidvigorousvortexing,asbufferscontaindetergent.Followingmixing,brieflypulseinamicrocentrifugetocollectmaterialfromcap.

Pleasenotethatthisproductcontains10%glycerolandmightappearasliquidbutisprovidedlyophilized.Allowtheproductseveralminutestosolubilizeafteraddingwater.MixthoroughlybutgentlyTakeextracaretomixthoroughlybeforeeachuse,astheproteinstendtosettlewiththemoredenselayerafterfreezing.

SelectedreferencesFraseretal.(2013).PhotophysiologicalandPhotosyntheticComplexChangesduringIronStarvationinSynechocystissp.PCC6803andSynechococcuselongatusPCC7942.PLoSONE8(3):e59861.doi:10.1371/journal.pone.0059861
Ryan-Keoghetal.(2012). IrondeficiencyincyanobacteriacausesmonomerizationofphotosystemItrimersandreducesthecapacityforstatetransitionsandtheeffectiveabsorptioncrosssectionofphotosystemIinvivo.J.ofPhycology,1:145-154.


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