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Agrisera/ATG7 | Autophagyrelated protein 7/AS13 2699/
productinformation
Background

 Autophagy-relatedprotein7functionsasanE1enzymeessentialformultisubstratessuchasGABARAPL1andATG12.FormsintermediateconjugateswithGABARAPL1.FormationofthefinalGABARAPL1-PEconjugateisessentialforautophagy.

Immunogen

AsyntheticpeptidecorrespondingtotheC-terminalofhumanAPG7proteinconjugatedtobluecarrierproteinhasbeenusedastheimmunogen.Thepeptideishomologouswiththecorrespondingsequencederived fromAPG7proteininmouse,rat,S.cerevisiae,Macacamulatta(monkey)andCanisfamiliaris(dog).

HostRabbit
ClonalityPolyclonal
Clone
PuritySerum
FormatLyophilized
Quantity100µl
ReconstitutionForreconstitutionpleaseadd100µlofsterilewater
Storage

Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat 4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles.

TestedapplicationsImmunohistochemistry(IHC),immunofluorescence(IF)
Relatedproducts
Additionalinformation
applicationinformation
Recommendeddilution

Adilutionof1:200to1:3000dilutionisrecommendedfortheseapplications.Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.

Expected|apparentMW
Confirmedreactivityhuman,rat
Predictedreactivity

notyettestedinotherspecies

Notreactivein
Additionalinformation

tobeaddedwhenavailable

Selectedreferences

tobeaddedwhenavailable


Applicationexample





ConfocalmicroscopyonimmunofluorescentlydetectedAPG7incytospin-isolatedhumanwhitebloodcellsusingRabbitantibodytoATG7:wholeserum(AS132699)atadilutionof1:100,incubatedfor1hatroomtemperature.Thecellsweredouble-stainedforAPG7appearinginredandMyeloperoxidase(MPO)appearingingreen.ThecellswerethencounterstainedwithHoechstDye(bluecolour).Here,themergedpictureispresented.





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