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Agrisera/Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/fcomplex/AS06 119/188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Agrisera/Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/fcomplex/AS06 119/
productinformation
Background

Multi-subunitcomplexofcytb6/fisacrucialcomponentforthephotosyntheticelectrontransportchainofhigherplants,greenalgaeandcyanobacteria.Thiscomplexiscatalyzingoxidationofquinolsandthereductionthereductionofplastocyanin.ThisreactionallowstoestablishtheprotonforcerequiredfortheATPsynthesis.Fourmajorsubunitsbuildthecomplex:thepetAgeneproductcorrespondingtoac-typecytochrome(cytf),thepetBgeneproductcorrespondingtoab-type/c’-typecytochromewiththreehaems(cytb6),thepetDgeneproduct(subunitIV,orsuIV),andthepetCgeneproduct,correspondingtotheRieske/Iron/sulfurprotein.

Immunogen

GSTfusiontocytochromeffromChlamydomonasreinhartiiP23577

HostRabbit
ClonalityPolyclonal
Clone
PuritySerum
FormatLyophilized
Quantity50µl
ReconstitutionForreconstitutionadd50µlofsterilewater.
StorageStorelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.
TestedapplicationsWesternBlot(WB)
Relatedproducts

AS08306|anti-cytochromef(withreactivitytocyanobacteria)

CollectionofantibodiestoArabidopsisproteins

CollectionofantibodiestoChlamydomonasproteins

Plantandalgalproteinextractionbuffer

Secondaryantibodies

AdditionalinformationContains0.02%sodiumazideaspreservative.
applicationinformation
Recommendeddilution1:2000withalkalinephosphatase,1:50000withECL(WB)
Expected|apparentMW

34|31-32kDa

ConfirmedreactivityArabidopsisthaliana,Chlamydomonasreinhardtii,ColobanthusquitensisKuntBartl,Craterostigmapumilum,Hordeumvulgare,Nannochloropsisgaditana,Nicotianatabacum,Physcomitrellapatens,Selaginellamartensi,Synechocystis6803substrainPCC-M
PredictedreactivityCitrusxlimon,greenalgae,Zeamays
Notreactiveinnoconfirmedexceptionsfrompredictedreactivityknowninthemoment
AdditionalinformationReactionwithcyanobacteria:Synechocystis6803andSynechococcus7942possibletoobtainontotalcellextractwhenusingantibodyat1:500andlongerexposuretime.
SelectedreferencesGeorgetal.(2017).AcclimationofOxygenicPhotosynthesistoIronStarvationIsControlledbythesRNAIsaR1.CurrBiol.2017May22;27(10):1425-1436.e7.doi:10.1016/j.cub.2017.04.010.(SynechocystisPCC6803)Tyuerevaetal.(2017).TheabsenceofchlorophyllbaffectslateralmobilityofphotosyntheticcomplexesandlipidsingranamembranesofArabidopsisandbarleychlorinamutants.PhotosynthRes.2017Apr5.doi:10.1007/s11120-017-0376-9.(Hordeumvulgare,westernblot)
Ferronietal.(2016).LightacclimationinthelycophyteSelaginellamartensiidependsonchangesintheamountofphotosystemsandontheflexibilityofthelight-harvestingcomplexIIantennaassociationwithbothphotosystems.NewPhytol.2016Apr5.doi:10.1111/nph.13939.
Suorsaetal.(2015).Lightacclimationinvolvesdynamicre-organisationofthepigment-proteinmegacomplexesinnon-appressedthylakoiddomains.PlantJ.2015Aug29.doi:10.1111/tpj.13004.
Charuvietal.(2015).PhotoprotectionConferredbyChangesinPhotosyntheticProteinLevelsandOrganizationduringDehydrationofaHomoiochlorophyllousResurrectionPlant.PlantPhysiol.2015Apr;167(4):1554-65.doi:10.1104/pp.114.255794.
Hojkaetal.(2014).Induciblerepressionofnuclear-encodedsubunitsofthecytochromeb6fcomplexintobaccorevealsanextraordinarilylonglifetimeofthecomplex.PlantPhysiol.2014Jun24.pii:pp.114.243741.
Dangetal.(2014).CombinedIncreasesinMitochondrialCooperationandOxygenPhotoreductionCompensateforDeficiencyinCyclicElectronFlowinChlamydomonasreinhardtii.PlantCell.2014Jul2.pii:tpc.114.126375.

applicationexample

 

Chlamydomonasreinhardtiisamplesarelysedcells,Arabidopsisthalianasamplesarepreparedfromthylakoidmembranes;Sampleswereboiled1minin3%SDS,then10ugofchlorophyllswereloadedineachwellofa12%acrylamidegel.

 

Western blot detection using anti-cytochrome f antibodies


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