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Agrisera/sll0528 | Putative zinc metalloprotease sII0528/AS10 1592/188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Agrisera/sll0528 | Putative zinc metalloprotease sII0528/AS10 1592/
productinformation
BackgroundSll0528isatransmembranemetallo-proteasefromSynechocystissp.PCC6803.ThisproteasebelongstoSP2-typemetallopeptidases,thekeyplayerofintra-membraneproteolysis.
ImmunogenSyntheticpeptide(aminoacids271-286)specificforproteaseSll0528,Q55518fromSynechocystissp.PCC6803
HostRabbit
ClonalityPolyclonal
Clone
PuritySerum
FormatLiquid
Quantity100µl
Reconstitution
StorageShortterm4°C.Longterm-20°C.Repeatedfreezingandthawingisnotrecommended. Solutioncontains0.01%sodiumazide.
Testedapplicationswesternblot(WB)
Relatedproducts

AS101623|Anti-sll0528|putativezincmetalloproteasesII0528,largerpacksize

AS101596|Anti-slr1821|putativezincmetalloproteaseslr1821

Secondaryantibodies

AdditionalinformationPre-immuneserumforthisproductisavailableuponrequest.
applicationinformation
Recommendeddilution1:2000withstandardECL(WB)
Expected|apparentMW

40.5kDa

ConfirmedreactivitySynechocystissp.PCC6803
PredictedreactivityCrocosphaerawatsonii,Synechococcus,Microcystisaeruginosa,Nostocsp.
Notreactiveinnoconfirmedexceptionsfrompredictedreactivityknownatthemoment
Additionalinformationtobeaddedwhenavailable
Selectedreferencestobeaddedwhenavailable

applicationexample

western blot using anti-sll0528 antibodiesTotalproteinfromSynechocystissp.PCC6803wereseparatedon12%SDS-PAGE andblotted1htoPVDFusingsemi-drytransfer.Blotswereblockedwith 5%non-fatmilkfor1hatroomtemperature(RT)withagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:2000for1hatRTwithagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflytwice,thenwashedoncefor15minand3timesfor5mininTBS-TatRTwithagitation.Blotwasincubatedinsecondaryantibody(anti-rabbitIgGhorseradishperoxidaseconjugated)dilutedto1:10000in for1hatRTwithagitation.Theblotwaswashedasaboveanddevelopedfor5minwithECLaccordingtothemanufacturersinstructions.



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