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Agrisera/ABA | Abscisic acid/AS06 195/
productinformation
Background

Abscisicacid(ABA)isaplanthormoneinvolvedindifferentphysiologicalresponsesasstimulationoftheclosureofstomata(waterstressbringsaboutanincreaseinABAsynthesis),iInhibitionofshootgrowth,andmanyothers.ABAshowntohavebothinhibitoryaswellasmanypromotingfunctions.

Immunogen

BSA-conjugatedabscisicacid(ABA)

HostRabbit
ClonalityPolyclonal
Clone
PurityTotalIgG
FormatLyophilizedinPBS,Phosphate10mmol/l;NaCl150mmol/latpH7.35
Quantity100µl
ReconstitutionForreconstitutionadd50µlofsterilewaterand50µlofglycerol
Storage

storelyophilized/reconstitutedat-20°C;thisaliquotecanbefreezedandthawedforuptofivetimesandshowedstabilityforatleast2years.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.

TestedapplicationsELISA(ELISA),immunolocalization(IL)
Relatedproducts

AS09446|ABA|abscisicacid(C1)(forimmunolocalization)

AS09422|ABA|abscisicacid(C1)(quantity1mg)

AS09447|anti-ABA|abscisicacid(C1)(quantity5mg)

collectionofantibodiestootherplanthormones

AdditionalinformationtotalIgGwaspurifiedbyammoniumsulfateprecipitationandadsorbedonproteincarrier
applicationinformation
Recommendeddilution

1:5000-1:10000(ELISA),1:100(IL)

Expected|apparentMW
ConfirmedreactivityEuphorbiapulcherrima
Predictedreactivity

dicotsandmonocots

Notreactivein

noconfirmedexceptionsfrompredictedreactivityknowninthemoment

Additionalinformation

fordetailedimmunolocalizationprotocolcheck"moreinformation"

Selectedreferences

tobeaddedwhenavailable


applicationexample

immunolocalization of ABA in plant tissue

ABAlocalizationin5daysoldrootsofArabidopsisthaliana.ABAsignalisshowninred,PIN2signalshowningreen,DAPIinblue.Thematerial hasbeenfixedwith4%EDACfor10minfollowedbyEDACandpara-formaldehyde.Tissuecleaninghasbeenperformedbeforeimmunolocalization.Rabbitanti-ABAprimaryantibodydilutedin1:400,ALEXA555conjugatedanti-rabbitsecondaryantibody,diluted1:700(Invitrogen).Co-stainingwithDAPIvisualizednucleus(bluecolor).

CourtesyDr.TarasPasternak,FreiburgUniversity,Germany


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