Agrisera/RPS1 | Ribosomal protein S1 (mitochondrial)/AS16 3835/


productinformation
Background		RPS1(RibosomalproteinS1)islocalizedinmitochondriaandinvolvedinproteintranslationprocess.Alternativename:Ribosomalproteinsmallsubunit1.
Immunogen		KLH-conjugatedsyntheticpeptidechosenfromOryzasativaRPS1proteinsequenceUniProt:Q2F963;LOC_Osm1g00500.1
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		100µl
Reconstitution		Forreconstitutionadd100µlofsterilewater.
Storage		Storeat-20°C;makealiquotstoavoidrepeatedfreeze-thawcycles.
Testedapplications		WesternBlot(WB)
Relatedproducts		AS132650 \|anti-L13-1\|60SribosomalproteinL13-1,rabbitantibodies AS09478\|anti-L13-1\|60SribosomalproteinL13-1,rabbitantibodies AS08331\|anti-L-30\|50SribosomalproteinL30,rabbitantibodies AS111738\|anti-Rpl1\|50SribosomalproteinL1,rabbitantibodies AS152876 \|anti-RPL2\|ribosomalproteinL2(chloroplastic),rabbitantibodies AS142780 \|anti-RPL33\|50SribosomalproteinL33,chloroplastic,rabbitantibodies AS142781 \|anti-RPL36\|50SribosomalproteinL36,chloroplastic,rabbitantibodies AS122115 \|anti-RPL37\|ribosomalproteinL37,cytoplasmic,rabbitantibodies AS152875 \|anti-RPS1\|ribosomalproteinS1(chloroplastic),rabbitantibodies AS122114 \|anti-RPS12\|ribosomalproteinS12,(chloroplastic),rabbitantibodies AS142779 \|anti-RPS15\|30SribosomalproteinS15,(chloroplastic),rabbitantibodies AS10719 \|anti-Rps2\|anti-ribosomalsubunit2(cytoplasmic),rabbitantibodies AS163832\|Anti-RPS2\|RibosomalproteinS2(mitochondrial),rabbitantibodies AS08309 \|anti-S1\|anti-30SribosomalproteinS1,rabbitantibodies AS122111 \|anti-S14\|40SribosomalproteinS14-1,rabbitantibodies AS153068\|anti-S4\| mitochondrialribosomalsmallsubunitproteinS4,rabbitantibodies AS153067\|anti-S10\| mitochondrialribosomalsmallsubunitproteinS10,rabbitantibodies AS153069\|anti-L16 mitochondrialribosomallargesubunitproteinL16,rabbitantibodies antibodiestoproteinsinvolvedintranslation
Additionalinformation

applicationinformation
Recommendeddilution		1:1000(WB)
Expected\|apparentMW		20kDa
Confirmedreactivity		Oryzasativa
Predictedreactivity		Oryzasativa
Notreactivein
Additionalinformation
Selectedreferences		Tobeaddedwhenavailable,antibodyreleasedinApril2016.

Applicationexample

western blot using anti-RPS1 (mitochondrial) rabbit antibodies

50-100µgoftotalproteinfromOryzasativaflagleaf,rootsorfringeextractedwithproteinextractionreagentwereseparatedon15%SDS-PAGEandblotted1htoPVDFusingwettransfer.Blotswereblockedwith1×TBST+5%Nonfatdrymilkfor1hat37°Cwithagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:1000in1×TBST+5%Nonfatdrymilkfor1hatRTwithagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflytwice,thenwashedoncefor15min.and3timesfor5min.inTBS-TatRTwithagitation.Blotwasincubatedinsecondaryantibody(anti-rabbitIgGHRP/conjugated)dilutedto1:15000in1xTBST+5%Nonfatdrymilkfor2hatRTwithagitation.Theblotwaswashedasaboveanddevelopedfor5min.withECLaccordingtothemanufacturersinstructions.Exposuretimewas10seconds.

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Agrisera/RPS1 | Ribosomal protein S1 (mitochondrial)/AS16 3835/