Agrisera/CAH3 | Carbonic anhydrase/AS05 073/


productinformation
Background		CAH3-carbonicanhydraseisazinc-containingmetalloenzymethatcatalyzesthereversibleinterconversionofCO₂andHCO₃–.Itplaysanimportantroleinmanyphysiologicalfunctionsthatinvolvedecarboxylationorcarboxylationreactions,includingbothphotosynthesisandrespiration.
Immunogen		RecombinantcarbonicanhydraseofChlamydomonasreinhardtii A8J4Z8aftercleavageofafusionprotein
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		100µl
Reconstitution		Forreconstitutionadd100µlofsterilewater.
Storage		storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.
Testedapplications		westernblot(WB),immunofluorescence(IF),immunogold(IG)
Relatedproducts		antibodiestootherphotosyntheticproteins CollectionofantibodiestoChlamydomonasproteins Algalproteinextractionbuffer Secondaryantibodies
Additionalinformation		thisantibodyrecognizesnativeandrecombinantcarbonicanhydraseofChlamydomonasreinhardtii

applicationinformation
Recommendeddilution		1:2000withstandardECL(WB),1:200(IF)
Expected\|apparentMW		33.4\|29kDa
Confirmedreactivity		Chlamydomonasreinhardtii
Predictedreactivity		Chlamydomonasreinhardtiionly
Notreactivein		Arabidopsisthaliana,Oryzasativa
Additionalinformation		tobeaddedwhenavailable
Selectedreferences		Correa-Galvisetal.(2016).PhotosystemIISubunitPsbSIsInvolvedintheInductionofLHCSRProtein-dependentEnergyDissipationinChlamydomonasreinhardtii.JBiolChem.2016Aug12;291(33):17478-87.doi:10.1074/jbc.M116.737312. Mitchelletal.(2014).Dynamicsofcarbonconcentratingmechanisminductionandproteinre-localisationduringthedarktolighttransitioninsynchronisedChlamydomonas.PlantPhysiol.2014Aug8.pii:pp.114.246918. Tirumanietal.(2014).RegulationofCCMgenesinChlamydomonasreinhardtiiduringconditionsoflight-darkcyclesinsynchronouscultures.PlantMolBiol.2014Mar4. Shutovaetal.(2008).ThephotosystemII-associatedCah3inChlamydomonasenhancestheO2evolutionratebyprotonremoval.EMBOJ.27:782-791.

applicationexample
western blot

(1)Chlamydomonasreinhardtiithylakoidpreparation,(2)overexoressedcarbonicanhydrase,primaryantibodyusedin1:2000dilution.Secondaryantibodiesanti-rabbitIgG(GEHealthcare).usedat1:10000withstandardECL

western

20µg/mlofchlorophyllfromChlamydomonasreinhardtiiCah3-lessmutant(1),and15µg/mlofchlorophyllfromChlamydomonasreinhardtiiwild-type(2),extractedwith4xLaemmliwereseparatedon12%SDS-PAGEandblotted2htonitrocellulosefilterusingtanktransfer.Blotswereblockedwith5%milkpowderforfor2hatroomtemperature(RT)withagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:1000fovernightat8°Cwithagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbriefly,thenwashedoncefor15minand3timesfor20mineachinTBS-TatRTwithagitation.Blotwasincubatedinsecondaryantibody(anti-rabbitIgGhorseradishperoxidaseconjugated,fromAgriseraAS09602)dilutedto1:20000infor1hatRTwithagitation.Theblotwaswashedasaboveanddevelopedfor1minwithECLaccordingtothemanufacturersinstructions.Exposuretimewas10min.MWstandardsused,Pageruler,pre-stainedfromThermofisher.

CourtesyofDr.GöranSamuelsson,UmeåPlantScienceCentre,Sweden

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Agrisera/CAH3 | Carbonic anhydrase/AS05 073/