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Agrisera/Urease, Biotin conjugated/AS09 560/
productinformation
Background

Urease(EC=3.5.1.5)isanenzymeinvolvedinnitrogenmetabolism.Itiscatalyzesthehydrolysisofureaintocarbondioxideandammonia.

Immunogen

ureaseisolatedandpurifiedfromCanavaliaensiformis(commonjack-beans)

HostRabbit
ClonalityPolyclonal
Clone
PurityPurifiedIgG
FormatLyophilizedinphosphatebuffersaline
Quantity1ml
ReconstitutionForreconstitutionpleaseadd1mlofsteriledestilledwater.Afterreconstitutionwith1mlwatertheIgGconcentrationintheproductis10mg/ml.
Storage

storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.

Testedapplicationswesternblot(WB),ELISA(ELISA),immunofluorescence(IF),immunohistochemistry(IHC)
Relatedproducts

AS09559|anti-urease

AS09561|anti-urease(affinitypurified)

Plantproteinextractionbuffer

Additionalinformation

AntibodyislabelledwithbiotinusingN-hydroxysuccinimidobiotin.Antibodypotencyandpurityhasbeenevaluatedbyimmunoelectrophoresis,singleradialimmunodiffusion(Ouchterlony),ELISA,immunoblottingandenzymeinhibition.

applicationinformation
Recommendeddilution

1:1000-1:30000fortechniqueslistedabove

Expected|apparentMW

91kDa

ConfirmedreactivityCanavaliaensiformis(commonjack-bean)
Predictedreactivity

dicotsincluding:Arabidopsisthaliana,Glycinemax,Ricinuscommunis

Notreactivein

noconfirmedexceptionsfrompredictedreactivityknowninthemoment

Additionalinformation

Biotin/IgGproteinmolarrationisapproximately6.2.Noforeignproteinsareadded.

Selectedreferences

tobeaddedwhenavailable



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