Agrisera/H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)/AS16 3195/


productinformation
Background		Histonemethylationanddemethylationisdynamicallyregulatedbyrespectivelyhistonemethyltransferasesandhistonedemethylases.AcetylationofhistoneH3K27isassociatedwithactivepromotersandenhancers.
Immunogen		KLH-conjugatedsyntheticpeptide
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		AffinitypurifiedseruminPBSwith0.05%sodiumazide
Format
Quantity		50µg
Reconstitution
Storage		Storeat-20°C;makealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfrommaterialadheringtothecaporsidesofthetubes.
Testedapplications		chromatinimmunoprecipiation(ChIP/ChIP-seq),Dotblot(Dot),ELISA(ELISA),immunofluorescence(IF),peptidearray(PA),westernblot(WB)
Relatedproducts		AS163190-10\|anti-H3K4me3\|histoneH3,trimethylatedlysine4(H3K4me3)(10µg),rabbitantibodies AS163187\|anti-H3K56me1\|HistoneH3(monomethylLys56),polyclonalantibodies AS163182\|anti-H3K18me2\|HistoneH3(dimethylLys18),rabbitantibodies AS163183\|anti-H3K18me3\|HistoneH3(trimethylLys18),rabbitantibodies AS163181\|anti-H3K18me1\|HistoneH3(monomethylLys18),rabbitantibodies AS163168\|anti-H3T3pK4me1\|HistoneH3(monomethylLys4,pThr3),rabbitantibodies AS163169\|anti-H3T3pK4me2\|HistoneH3(dimethylLys4,pThr3),rabbitantibodies AS163172\|anti-H3K4me3T6p\|H3(trimethylLys4,pThr6),rabbitantibodies AS163197\|anti-anti-H3core,monoclonalantibodies AS152855\|anti-H3\|histoneH3,chickenpolyclonalantibodies AS10710\|anti-H3\|histoneH3,rabbitpolyclonalantibodies AS10710PRE\|H3\|histoneH3,pre-immuneserumtoH3antibodyAS10710 collectionofantibodiestoepigenetics
Additionalinformation		AntibodyisprovidedinPBScontaining0.05%azideand0.05%ProClin300.

applicationinformation
Recommendeddilution		0.5-5µg/IP(ChIP/ChIP-seq),1:20000(Dot),1:500(ELISA),1:500(IF), 1:1000(WB)
Expected\|apparentMW		15kDa
Confirmedreactivity		human
Predictedreactivity		A.thaliana,mouse,rat
Notreactivein		Noconfirmedexceptionsfrompredictedreactivityknownatthemoment
Additionalinformation		AntibodysolutioncontainsPBSwith0.05%sodiumazideand0.05%ProClin300.
Selectedreferences		Tobeaddedwhenavailable,antibodyreleasedinFebruary2016.

applicationexample

Dot blot using anti-H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade) polyclonal antibodies

Dotblotwasusedtototestthecrossreactivityofanti-H3K27acantibodies.PeptidescontainingotherhistonemodificationsandtheunmodifiedH3K27wereused.100to0.2pmoloftherespectivepeptideswerespottedonamembrane.Theantibodywasusedatadilutionof1:20000.Resultsshowahighspecificityoftheantibodyforthemodificationofinterest.

新闻动态

行业前沿

技术文章

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Agrisera/H3K27ac | Histone H3 acetylated lysine 27 (ChIP grade)/AS16 3195/