Agrisera/AKING1 | SNF1related protein kinase regulatory subunit gamma 1/AS09 613/


productinformation
Background		AKING1isamemberofSnRK1/SNF1/AMPKfamilyinplants.ItisaregulatorysubunitoftheputativetrimericSNF1-relatedproteinkinase(SnRK)compleswhichmayplayaroleinasignaltransductioncascaderegulatinggeneexpressionandcarbohydratemetabolisminhigherplants.
Immunogen		KLH-conjugatedpeptidederivedfromArabidopsisthalianaAKING1Q8LBB2,At3g48530
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		AffinitypurifiedseruminPBSpH7.4.
Format		Lyophilized
Quantity		50µg
Reconstitution		Forreconstitutionadd50µlofsterilewater.
Storage		storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.
Testedapplications		westernblot(WB)
Relatedproducts		AS09460\|Anti-AKINB1\|SNF1-relatedproteinkinaseregulatorysubunitbeta-1,rabbitantibody AS09462\|Anti-AKINB2\|SNF1-relatedproteinkinaseregulatorysubunitbeta-2,rabbitantibody AS152859\|Anti-AKINß3\|SNF1-relatedproteinkinaseregulatorysubunitbeta-3,rabbitantibody AS09463\|Anti-AKINbetagamma,rabbitantibody AS10919\|Anti-AKIN10 SNF1-relatedproteinkinasecatalyticsubunitalphaKIN10,rabbitantibody AS10920\|Anti-AKIN11\|SNF1-relatedproteinkinasecatalyticsubunitalphaKIN11,rabbitantiboddy collectionofantibodiestoproteinsinvolvedinsignaltransduction Plantproteinextractionbuffer Secondaryantibodies
Additionalinformation

applicationinformation
Recommendeddilution		1:2000(WB)
Expected\|apparentMW		48kDa
Confirmedreactivity		Arabidopsisthaliana
Predictedreactivity		Arabidopsisthaliana
Notreactivein		Chlamydomonasreinhardtii,Vitisvinifera
Additionalinformation
Selectedreferences		Emanuelleetal.(2015).SnRK1fromArabidopsisthalianaisanatypicalAMPK.PlantJ.2015Mar3.doi:10.1111/tpj.12813. Ramonetal.(2013).ThehybridFour-CBS-DomainKINβγ-subunitfunctionsasthecanonicalγsubunitoftheplantenergysensorSnRK1.Plantj.April1.

applicationexample

western blot using anti-KIN gamma antibodies

10ugofArabidopsisthalianaColumbiaWTandKINgammaKOseedlingsweregrindedinMOPSloadingbufferandheatedfor5minat95°C.Sampleswereseparatedon10%SDS-PAGEandblotted1htoPVDF.Blotswereblockedwith5%milkfor1hatroomtemperature(RT)withagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:2000for2hatRTwithagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbriefly
twice,thenwashedoncefor15minand3timesfor5mininTBS-TatRTwithagitation.Blotwasincubatedinsecondaryantibody,dilutedto1:4000infor1hatRTwithagitation.
Theblotwaswashedasaboveanddevelopedfor5minwithECLaccordingtothemanufacturersinstructions.Exposuretimewas15seconds.

CourtesyofDr.MatthewRamon

新闻动态

行业前沿

技术文章

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Agrisera/AKING1 | SNF1related protein kinase regulatory subunit gamma 1/AS09 613/