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Agrisera/VDAC1 | Voltagedependent anionselective channel protein 1 (40 µg, ALPconjugated)/AS07 21188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Agrisera/VDAC1 | Voltagedependent anionselective channel protein 1 (40 µg, ALPconjugated)/AS07 21
productinformation
Background

VDAC1 protein(calledalsoSynonymes:At3g01280,outermitochondrialmembraneproteinporin1,T22N4_9,T22N4.9,VDAC1,Voltage-dependentanion-selectivechannelprotein1,voltage-gatedion-selectivechannel)formsachannelthroughthecellmembranefordiffusionofsmallhydrophilicmolecules.EvolutionaryoriginofVDACproteinisnotclearandtheirstructureandpropertiesarequitedifferentmakingthoseproteinsonlyconceptuallylikeporins(Clausenetal.2004).

Immunogen

KLH-conjugatedpeptideconservedinallknownhigherplantVDAC1proteinsincludingArabidopsisthalianawiththelocusname:At3g01280

HostRabbit
ClonalityPolyclonal
Clone
PurityAffinitypurifiedserum
FormatLiquidinPBSpH7.4,conjugatedtoALP
Quantity40µg
Reconstitution
Storage

Storeat+4°Cfor12-18months.Apreservativemaybeaddedforlongtimestorageupto2years.

Testedapplicationswesternblot(WB),Blue-native(2DBN/SDS-PAGE),immunolocalization(IL)
Relatedproducts

AS04054|anti-AOX1/2rabbitantibody,Markerofmitochondrialinnermembrane

AS06203A|anti-Idhrabbitantibody,markerofmitochondrialmatrix

collectionofantibodiestoothermitochondrialproteins

Additionalinformation

cellular[compartmentmarker]ofmitochondrialoutermembrane

applicationinformation
Recommendeddilution

1:5000on2-30µgofprotein/lanewithstandardECL(WB), 1:500(IL)

Expected|apparentMW

29kDa(forArabidopsisthaliana)

ConfirmedreactivityArabidopsisthaliana,Betavulgaris,Brassicaoleraceavar.botrytis,Oryzasativa,Papaversp.pollentubes(IL),Spinaciaoleracea,Physcomitrellapatens
Predictedreactivity

Arabidopsisalpina,Aundodonax,Brachypodiumdistachyon,Brassicacampestris,Brassicanapus,Brassicarapasubsp.pekinensis, Capsellarubella,Citrusclementina,Citrussinensis,Eutremasalsugineum,Glycinemax,Glycinesoja, Gossypiumarboreum,Hoedumvulgarevar.distichum, Jatrophacurcas,Medicagotruncatula,Mesembryanthemumcrystallinum, Morusnotabilis,Nicotianatabacum,Phaseoluscoccineus,Phaseolusvulgaris, Pisumsativum,Plantagomajor,Prunuspersica, Ricinuscommunis,Solanumlycopersicum,Solanumtuberosum, Sorghumbicolor,Theobromacacao,Triticumaestivum,Vitisvinifera,Zeamays

Notreactivein

Chlamydomonasreinhardtii,Glycinemax,Zeamays,diatoms,Saccharomycescerevisiae

Additionalinformation

Amountofmitochondrialfractiondetectedbyanti-VDAC1antibodywasfrom2-10µg.

Immunolocalizationmethoddescriptionandimagesareavailablehere

Blue-native(2DBN/SDS-PAGE)methodologyisdescribedinPiechotaetal.2010

Selectedreferences


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