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Agrisera/HSP90 | Heat shock protein 90/AS05 063/
productinformation
Background

HSP90isaubiquitouschaperoneinvolvedinnumerouscellularprocesses.Membersofthisfamilyofproteinsplayimportantrolesinallowingaselectedgroupofintracellularsignalingmoleculesreachandmaintainfunctionallyactiveconformations.Alternativenames:forhsp90alpha:HSP86,renalcarcinomaantigenNY-REN-38,forhsp90beta:hsp84

Immunogen

KLH-conjugatedsyntheticpeptidechosenfromahighlyconservedregionofhsp90foundinboththealphaP07900andbetaP08238formoftheprotein.Thetargetpeptideisperfectlyconservedinanimals.

HostRabbit
ClonalityPolyclonal
Clone
PuritySerum
FormatLyophilized
Quantity100µl
ReconstitutionForreconstitutionadd100µlofsterilewater
Storage

storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.

TestedapplicationsWesternblot(WB)
Relatedproducts

AS05061|anti-salmonidHSP70

AS05061A|anti-salmonidHSP70,suitableforimmunoprecipitation

AS05062|anti-salmonidHSC70

AS05083A|anti-HSP70/HSC70globalantibody,affinitypurified

Secondaryantibodies

Additionalinformation
applicationinformation
Recommendeddilution

1:5000withstandardECL(WB)

Expected|apparentMW

84-86kDa

Confirmedreactivitysalmon,human
Predictedreactivity

fishes,hen,mammals

Notreactivein

noconfirmedexceptionsfrompredictedreactivityknowninthemoment

Additionalinformation

insalmonidfishacross-reactivebandatapproximately40kDaisobserved;antibodywillalsodetectahumanrecombinantHSP90protein

AntibodyisreactingstronglywithrecombinantHSP90fromHELAcells. 

SelectedreferencesRickettsetal.(2015).TheEffectsofAcuteWaterborneExposuretoSublethalConcentrationsofMolybdenumontheStressResponseinRainbowTrout,Oncorhynchusmykiss.PLoSOne.2015Jan28;10(1):e0115334.doi:10.1371/journal.pone.0115334.eCollection2015.
Liuetal.(2014).SpermidineEnhancesWaterloggingToleranceviaRegulationofAntioxidantDefence,HeatShockProteinExpressionandPlasmaMembraneH+-ATPaseActivityinZeamays.J.AgronomyandCropScience,Articlefirstpublishedonline:1APR2014,DOI:10.1111/jac.12058.
Chandraetal.(2012).Sustainedhightemperatureincreasesthevitellogeninresponseto17alpha-ethynylestradiolinmummichog(Fundulusheteroclitus).Aquatictoxicology.

applicationexample-Westernblot:

 


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