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Agrisera/FIE | Fertilizationindependent endosperm/AS12 2616/

 



productinformation
BackgroundFIE(fertilization-independentendosperm)belongstopolycombgroup(PcG)proteinwhichactbyformingmultiproteincomplexes,requiredtomaintainthetranscriptionallyrepressivestateofhomeoticgenesthroughoutdevelopment.PcGproteinsarenotrequiredtoinitiaterepression,buttomaintainitduringlaterstagesofdevelopment. 
Immunogen

KLH-conjugatedsyntheticpeptidederivedfromArabidopsisthaliana FIEproteinsequence,UniProt:Q9LT47,TAIR:At3g20740

HostRabbit
ClonalityPolyclonal
Clone
PurityAffinitypurifiedserum
FormatLyophilizedinPBSpH7.4
Quantity50µg
ReconstitutionForreconstitutionadd50µlofsterilewater.
Storage

storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.

Testedapplicationswesternblot(WB)
Relatedproducts

collectionofantibodiestoproteinsinvolvedinplantdevelopment

Plantproteinextractionbuffer

Secondaryantibodies

Additionalinformation
applicationinformation
Recommendeddilution

1:1000withECL(WB)

Expected|apparentMW

41kDa

ConfirmedreactivityRecombinantFIEprotein
PredictedreactivityArabidopsisthaliana,Triticumaestivum
Notreactivein

noconfirmedexceptionsfrompredictedreactivityarecurrentlyknown

Additionalinformation

ThisantibodysofarhasbeenconfirmedtodetectrecombinantFIEprotein,GFP-tagged. 

OptimizationofproteinextractionanddetectionusingmostsensitivereagentsmayresultinrecognitionofendogenousFIE.

Selectedreferences

tobeaddedwhenavailable,antibodyreleasedinMarch2016. 


 


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