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Agrisera/mCherry/AS16 3967/

productinformation
BackgroundmCherryisderivedfromDsRed,aredfluorescentproteinfromso-calleddisccoralsofthegenusDiscosoma.DsRedisa223aminoacid~28kDaproteinsimilarinsizeandpropertiestoGFP,hoeveritproducesaredratherthanagreenfluorochrome.mCherryinitsmonomericformisusefulforapplicationssuchasFörsterResonanceEnergyTransfer(FRET,alsoknownasFluorescenceResonanceEnergyTransfer).TheproteinisanengineeredderivativeofoneofafamilyofproteinsoriginallyisolatedfromCnidarians(jellyfish,seaanemonesandcorals).
ImmunogenRecombinantfulllengthmCherryexpressedandpurifiedfromE.coli.
HostMouse
ClonalityMonoclonal
CloneIgG2a
PurityPurifiedfromtissueculturesupernatant.
FormatLyophilized
Quantity100µg
ReconstitutionForreconstitutionadd100µlofsterilewater
Storage

Storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.

TestedapplicationsImmunocytochemistry(IC),WesternBlot(WB)
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AS152999|GFP(totalIgG),rabbitantibody

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AS152998|GFP(totalIgY), chickenantibody


AS15 3002| GFP(purifiedrecombinantprotein)

othertagantibodies

Additionalinformation
applicationinformation
Recommendeddilution1:250-1:500(IC),1:1000-1:2000(WB)
Expected|apparentMW
ConfirmedreactivitymCherry
Predictedreactivity
Notreactivein
AdditionalinformationInwesternblot~28kDabandisdetectedcorrespondingtointactfull-lengthmCherryonHEK293cellstransfectedwithmCherryvector. 
Selectedreferences

tobeaddedwhenavailable,antibodyreleasedin August2016. 




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