Agrisera/H3K9ac | Histone H3, acetylated lysine 9/AS16 3198/


productinformation
Background		Histonemethylationanddemethylationisdynamicallyregulatedbyrespectivelyhistonemethyltransferasesandhistonedemethylases.
Immunogen		KLH-conjugatedsyntheticpeptide
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		AffinitypurifiedseruminPBSwith0.05%sodiumazide
Format
Quantity		50µg
Reconstitution
Storage		Storeat-20°C;makealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfrommaterialadheringtothecaporsidesofthetubes.
Testedapplications		chromatinimmunoprecipiation(ChIP/ChIPseq),Dotblot(Dot),ELISA(ELISA),immunofluorescence(IF),westernblot(WB)
Relatedproducts		AS163195\|anti-H3K27ac\|HistoneH3acetylatedlysine27(ChIPgrade),rabbitantibodies AS163190-10\|anti-H3K4me3\|histoneH3,trimethylatedlysine4(H3K4me3)(10µg),rabbitantibodies AS163187\|anti-H3K56me1\|HistoneH3(monomethylLys56),polyclonalantibodies AS163182\|anti-H3K18me2\|HistoneH3(dimethylLys18),rabbitantibodies AS163183\|anti-H3K18me3\|HistoneH3(trimethylLys18),rabbitantibodies AS163181\|anti-H3K18me1\|HistoneH3(monomethylLys18),rabbitantibodies AS163168\|anti-H3T3pK4me1\|HistoneH3(monomethylLys4,pThr3),rabbitantibodies AS163169\|anti-H3T3pK4me2\|HistoneH3(dimethylLys4,pThr3),rabbitantibodies AS163172\|anti-H3K4me3T6p\|H3(trimethylLys4,pThr6),rabbitantibodies AS152855\|anti-H3\|histoneH3,chickenpolyclonalantibodies AS10710\|anti-H3\|histoneH3,rabbitpolyclonalantibodies AS10710PRE\|H3\|histoneH3,pre-immuneserumtoH3antibodyAS10710 collectionofantibodiestoepigenetics
Additionalinformation		AntibodyisprovidedinPBScontaining0.05%azideand0.05%ProClin300.

applicationinformation
Recommendeddilution		2-5µg/IP(ChIP),1:20000(Dot), 1:1000(ELISA),1:500(IF),1:1000 (WB)
Expected\|apparentMW
Confirmedreactivity		human
Predictedreactivity		mouse,P.falciparum,Populussp.
Notreactivein		Noconfirmedexceptionsfrompredictedreactivityknownatthemoment
Additionalinformation		Tobeaddedwhenavailable
Selectedreferences		Tobeaddedwhenavailable,antibodyreleasedinFebruary2016.

applicationexample

western blot using anti-H3K9ac | Histone H3, acetylated lysine 9 polyclonal antibodies

DotBlotanalysiswasperformedtotestthecrossreactivityofanti-H3K9acantibodieswithpeptidescontainingotherhistonemodificationsandtheunmodifiedH3K9sequence.100to0.2pmoloftherespectivepeptideswerespottedonamembrane.Theantibodywasusedatadilutionof1:20,000.Figureshowsahighspecificityoftheantibodyforthemodificationofinterest.TheantibodyrecognizestheH3K9acetylation,bothinthepresenceandtheabsenceoftheK14acetylation

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Agrisera/H3K9ac | Histone H3, acetylated lysine 9/AS16 3198/