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Agrisera/Bromelain, Biotin conjugated/AS09 553/
productinformation
Background

Bromelain(EC=3.4.22.32)isacysteineproteaseisolatedfrompineapplestem.

Immunogen

nativebromelainisolatedandpurifiedfromAnanascomosus

HostRabbit
ClonalityPolyclonal
Clone
PurityPurifiedIgG
FormatLyophilizedinphosphatebuffersaline
Quantity1ml
ReconstitutionForreconstitutionpleaseadd1mlofsteriledestilledwater.
Storage

storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.

Testedapplicationswesternblot(WB),ELISA(ELISA),immunofluorescence(IF),immunohistochemistry(IHC)
Relatedproducts

AS09552|anti-bromelain

AS09554|anti-bromelain(affinitypurified)

AS09546|anti-PPI|papain(biotinylated)

AS09547|anti-PPI|papain(affinitypurified)

Plantproteinextractionbuffer

Additionalinformation

AntibodyislabelledwithbiotinusingN-hydroxysuccinimidobiotin.Antibodypotencyandpurityhasbeenevaluatedbyimmunoelectrophoresis,singleradialimmunodiffusion(Ouchterlony),ELISA,immunoblottingandenzymeinhibition.

applicationinformation
Recommendeddilution

1:5000-1:50000fortechniqueslistedabove

Expected|apparentMW
ConfirmedreactivityAnanascomosus
Predictedreactivity

Ananascomosus

Notreactivein

noconfirmedexceptionsfrompredictedreactivityknowninthemoment

Additionalinformation

Biotin/IgGproteinmolarrationisapproximately6.2.Noforeignproteinsareadded.

Selectedreferences

tobeaddedwhenavailable



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