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Kapa biosystems/KAPA Stranded RNA-Seq with RiboErase/KK8711/12 adapters x 40 µl each

Description:

KAPASingle-IndexedAdapterKit,SetA(1.5µM)

KAPAStrandedRNA-SeqwithRiboErase

Evolvedtofocus.

TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.

  • Upto99.98%rRNAdepletion*
  • FlexIBLeinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
  • Robustandreproducibleacrossvariousinputamounts
  • Automation-friendlyworkflow

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

IndustryleADIngrRNAdepletion

  • SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
  • MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest

Unsurpassedsequencingdataquality

  • Detectionofmoregenesanduniquetranscripts
  • Accurateandclearidentificationofsplicesitesandalternativegenesplicing
  • ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts

Highlyreproduciblesequencingresults

  • Highcorrelationevenbetweendifferenttestingconditions
  • Lowsample-to-samplevariationformorereliableresults

Improvedcoverageuniformity

  • Uniformdistributionofreadsovereachtranscript
  • Minimal5’–3’biasacrosstranscripts


*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

KAPA RNA HyperPrep Kits with RiboErase

KAPARNAHyperPrepKitswithRiboErase

KAPA mRNA HyperPrep Kits

KAPAmRNAHyperPrepKits

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

Library Quantification

KAPALibraryQuantificationKits

Applications:

Applications
  • WholeTranscriptome
  • GeneExpression
  • TranscriptDiscoveryandAnnotation
  • TotalRNA-Seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto10monthsat-20ºC.

KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).

Components

RIBO-Component Chart

Specifications

Spec
Description
CompatiblePlatform
IlluminaHiSeq®,NextSeq®andMiSeq®
LibraryType
RNA
StartingMaterial
High-qualityTotalRNA
InputAmount
100ng-1µg
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