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Kapa biosystems/KAPA Stranded RNA-Seq with RiboErase/KK8711/12 adapters x 40 µl each

Description:

KAPASingle-IndexedAdapterKit,SetA(1.5µM)

KAPAStrandedRNA-SeqwithRiboErase

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TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.

Upto99.98%rRNAdepletion*
FlexIBLeinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
Robustandreproducibleacrossvariousinputamounts
Automation-friendlyworkflow

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

_*Dataonfile.ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

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IndustryleADIngrRNAdepletion

SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest

High-quality sequencing data obtained from KAPA Stranded RNA-Seq Kit with RiboErase when compared to the equivalent Illumina or NEB kits. With equivalent mapping rates, KAPA Stranded RNA-Seq Kit with RiboErase provides lower duplication rates, better coverage uniformity and consistently detects more unique transcripts and genes. This enables a more sensitive detection of the expressed genes within the sample. Data on file.

Improved coverage of GC-rich transcripts. IGV image showing coverage and splice junction tracks of the haemoglobin alpha transcript (HBA1) which has a GC content of 62.8%. The GC content track shows GC-rich regions in red, and AT-rich regions in blue. More even coverage, improved splice-site recognition, and more representative plot compared to the undepleted sample (red) is seen when libraries are prepared with the KAPA Stranded RNA-Seq Kit with RiboErase (green) compared to the equivalent Illumina (orange) or NEB (blue) kits. Data on file.

Better detection of low-abundance transcripts. IGV image showing coverage and splice junction tracks of MALAT1, a low abundance long non-coding RNA transcript, which is more comprehensively covered with use of KAPA Stranded RNA-Seq Kit with RiboErase (green) for library preparation. In comparison, Illumina Ribo-Zero Gold (orange) and NEBNext (blue) show less even coverage of this transcript. Data on file.

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Unsurpassedsequencingdataquality

Detectionofmoregenesanduniquetranscripts
Accurateandclearidentificationofsplicesitesandalternativegenesplicing
ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts

Hierarchically clustered heatmap showing the correlations of gene expression levels between different laboratories and RNA input amounts. All correlations were greater than 0.93, with technical replicates produced in the same lab exceeding 0.98. Generally, all libraries from a single lab, including those produced from different input amounts, clustered together. The data indicates that the KAPA Stranded RNA-Seq Kit with RiboErase delivers robust, reproducible, and unbiased gene expression data from a wide range of input amounts. Data on file.

0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile."> Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.

Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.

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Highlyreproduciblesequencingresults

Highcorrelationevenbetweendifferenttestingconditions
Lowsample-to-samplevariationformorereliableresults

Improved coverage of the whole transcript. The housekeeping gene Beta-Actin has improved coverage at the 3’ and 5’ regions using the KAPA Stranded RNA-Seq Kit with RiboErase (green) when compared to the equivalent Illumina Ribo-Zero Gold (orange) and NEBNext (blue) kits. Data on file.

Minimal positional bias. With 100 ng of high-quality RNA input, 3

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Improvedcoverageuniformity

Uniformdistributionofreadsovereachtranscript
Minimal5’–3’biasacrosstranscripts

_*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAStrandedmRNA-SeqKits

KAPARNAHyperPrepKitswithRiboErase

KAPAmRNAHyperPrepKits

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits

Applications:

Applications

WholeTranscriptome
GeneExpression
TranscriptDiscoveryandAnnotation
TotalRNA-Seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto10monthsat-20ºC.

KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).

Components

Specifications

CompatiblePlatform

IlluminaHiSeq®,NextSeq®andMiSeq®

LibraryType

RNA

StartingMaterial

High-qualityTotalRNA

InputAmount

100ng-1µg

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Kapa biosystems/KAPA Stranded RNA-Seq with RiboErase/KK8711/12 adapters x 40 µl each

Description:

KAPAStrandedRNA-SeqwithRiboErase

Evolvedtofocus.

ProductHighlights

IndustryleADIngrRNAdepletion

Unsurpassedsequencingdataquality

Highlyreproduciblesequencingresults

Improvedcoverageuniformity

RelatedProducts

Applications:

KitSpecificationsandContents/Storage:

Components

Specifications