Product Description
CleanPlex® for MGI SARS-CoV-2 Panel for COVID-19 coronavirus detection, tracking, and research via amplicon-based target enrichment for Next Generation Sequencing (NGS) on MGI Tech’s DNBSEQ platforms
Paragon Genomics designed a highly multiplexed target enrichment panel covering the entire genome of SARS-CoV-2 virus (except for 92 bases at the ends). The panel enables complete genome sequencing and epidemiological studies of the new SARS-CoV-2 virus responsible for the COVID-19 pandemic. With Paragon Genomics CleanPlex technology, the entire genome of the virus can be amplified from RNA to sequence-ready libraries in 5 hours. Two versions of this the panel are available for two major sequencing platforms: Illumina and MGI. CleanPlex Technology allows the ease and flexibility to sequence samples for confident infectious disease surveillance and research.
Highlights
- Significant Cost Savings 98% reduction in sequencing cost. Only 0.2M reads per sample required v.s. 10M reads required by many shotgun metagenomics sequencing methods.
- High Coverage of Target Regions 99% coverage of the entire SARS-CoV-2 genome
- Sensitive Detection Can detect down to 1 copy with high confidence compared 3-5 copies by RT-qPCR (1)
- Fast, Streamlined Workflow Generate sequencing-ready libraries in just 5.5 hours using a rapid, four-step protocol including RT step with minimal hands on time.
- Superb Performance Prepare high-quality NGS libraries with excellent on-target performance using CleanPlex Technology to enable efficient use of sequencing reads. CleanPlex panels’ on-target rates are usually much higher than those of hybrid captured-based small target enrichment panels.
The CleanPlex SARS-CoV-2 Panel contains the CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit with RT components. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads are ordered separately to complete the workflow from input RNA to sequencing-ready NGS libraries.
Storage Temperature
Store at -20 °C.
(1) Corman VM et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988269/
For Research Use Only. Not for use in diagnostic procedures.
(Left) Using plasmids containing the N and S genes of SARS-CoV-2 on with a single pool workflow for detection, the detection rate at low copy numbers are show. The detection rate was shown to be 56% at 1.1 copy and 100% when more than 2 copies were present. With the use of both pools to cover the entire viral genome, the sensitivity is expected to only further increase. (Right) For these detected, the panel was able to on average capture all targets within 10 fold read depth range for 2.8 viral copies, and similarly for all but one target for 1.4 viral copies. All targets were also uniformly distributed across the GC range.