Lucerna/DFHBI1T 1 mg/4101mg/1mg

OTHERTOOLSANDRESOURCES  

 

 

 

CHEMICALSTRUCTURE

 

 

 

(Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4H)-one (DFHBI-1T) 

 

 

MOLECULARFORMULA:

C13H9F5N2O2

 

MOLECULARWEIGHT:

320.21

 

CASNAME/NUMBER:

4H-Imidazol-4-one,5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-,(5Z)/1539318-36-0

 

 

 

  

FLUORESCENCESPECTRA

 

AbsorptionandfluorescenceemissionspectraofSpinach2TM/DFHBI-1TinpH7.4buffer.

 

 

PRESENTATION: Lyophilizeddye

  

 
STORAGECONDITIONS: Stableinthedarkfor2yearat-20ºC. 

 

 

QUALITYASSURANCE: Productsareanalyzedby1HNMRandLC-MSandprovidedatpurityof>95%byHPLC. 

 

 

USAGESTATEMENT: Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureoffluorophoresandaptamers,wecannotpredictorbeheldresponsiblewithrespecttohowLucernaTM productswillbehaveinitscustomerssystems.ResearchersusingLucernaTMproductsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication. 

 

 

 

 

DFHBI-1TIMAGES 

 

 

A.Live-cellimagingofaCOS7cellexpressingCGG60-Spinach2TM inthepresenceofeither20µMDFHBIorDFHBI-1T. Imagesofthesamecellwereacquiredusinga100msecexposurewithaGFPfilterset. B.Quantificationofaverage DFHBI-1Tbrightnessof10cellsexpressingCGG60-SpinachTM normalizedtothebrightnessofDFHBI.

  

 

REFERENCEFORTHEPRODUCT:

 

SongW,StrackRL,SvensenN,JaffreySR.2014. Plug-and-PlayFluorophoresExtendtheSpectral PropertiesofSpinach.JACS136(4):1198-1201.

 

 

 

THISPRODUCTWASCITEDIN: 

 

SvensenN,JaffreySR.2016. FluorescentRNAaptamersasatooltostudyRNA-modifyingenzymes. CellChemBiol23(3):415-25.

 

AwSS,Tang,MX,TeoYN,CohenSM.2016.Aconformation-inducedfluorescencemethodformicroRNAdetection. NucleicAcidsRes44(10):e92.

 

AlamKK,TawiahKD,LichteMF,Porciani,BurkeDH.2017.AfluorescentsplitaptamerforvisualizingRNA-RNAassemblyinvivo. ACSSynthBiol6(9):1710-1721.

 

 

LICENSING: 

 

Lucernahastheexclusiverightstoallpatentscoveringtheuseofthisproduct. AllcommercialusersshouldcontactLucernaforauselicense. 

 


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