产品资料

Lucerna/Diglycyl-Lysine Antibody, clone GX41 (1 mg)/30-1000/1000

OTHERTOOLSANDRESOURCES

REFERENCEFORTHEPRODUCT:

Globalanalysisoflysineubiquitinationbyubiquitinremnantimmunoaffinityprofiling. XuG,PaigeJS,JaffreySR.NatureBiotechnology. 2010Aug;28(8)868-73.

THISPRODUCTWASCITEDIN:

A proteome-wide, quantitative survey ofinvivo ubiquitylation sites reveals widespread regulatory roles. WagnerSA, etal. Molecular&CellularProteomics. 2011Sep.

Proteomicanalysesrevealdivergentubiquitylationsitepatternsinmurinetissues.WagnerSA,etal. Molecular&CellularProteomics.2012Jul.

Systems-wideanalysisofubiquitylationdynamicsrevealsakeyroleforPAF15ubiquitylationinDNA-damagebypass.PovlsenLK,etal. NatureCellBIOLOGy. 2012Sep.

SpeciesReactivity	KeyApplications	Host	Format	AntibodyType
Human,mouse,rat,chicken,yeast,cowhorse	WB,IP	Mouse	Purified	MonoclonalAntibody

DESCRIPTION:Anti-Diglycyl-lysineantibody,cloneGX41

SPECIFICITY:Antibodyrecognizeslysineresiduesmodifiedbydiglycine,anadductleftatsitesofubiquitinationaftertrypsindigestions.

SOURCE/PURIFICATION:Monoclonalantibodiesareproducedbyimmunizinganimalswithasyntheticdiglycine-modifiedhistonepeptideandestablishedinhybridomalineGX41.AntibodiesarepurifiedbyproteinAandpeptideaffinitychromatography.

PRESENTATION:LyophilizedIgG

STORAGECONDITIONS:Stablefor2yearat-20ºC.

QUALITYASSURANCE:EachantibodybatchwillbemeasuredforitsABIlitytobindtoproteinscontainingdiglycyl-lysineandtorecoverdiglycyl-lysine-containingpeptidesfromtrypticdigestsofHEK293cells.

USAGESTATEMENT:Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureofantibodiesandantigens,wecannotpredictorbeheldresponsIBLewithrespecttohowLucerna^TMproductswillbehaveinitscustomers"systems.ResearchersusingLucerna^TMproductsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication.

LICENSING:CornellUniversityhasfiledausepatentonproteomicmethodsthatusethediglycyl-lysineantibodytoidentifyubiquitinatedproteins.Allcommercial usersshouldcontactCornellUniversityforauselicense.

GX41antibodyrecognizesthediglycyl-lysinemoietyatubiquitinatedsites.

Specificityofanti-diglycyl-lysine(cloneGX41)isverifiedbywesternblotanalysisofbeta-lactoglobulin,lysozyme,orratbrainlysate,inwhichthelysineswereeitherunmodified(A)ormodifiedwithBoc-Gly-Gly(B),respectively.

Equimolaramountsofthetwopeptides(GGDRVYIHPFHLandAc-SYSMEHFRWGK*PV-NH2)wereimmunoprecipitatedwithimmobolizedGX41antibodyandanalyzedbyMALDI-TOFMS.

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Lucerna/Diglycyl-Lysine Antibody, clone GX41 (1 mg)/30-1000/1000