产品资料

Lucerna/Diglycyl-Lysine Antibody, clone GX41 (1 mg)/30-1000/1000

OTHERTOOLSANDRESOURCES 

 

 

 

REFERENCEFORTHEPRODUCT: 

 

Globalanalysisoflysineubiquitinationbyubiquitinremnantimmunoaffinityprofiling. XuG,PaigeJS,JaffreySR.NatureBiotechnology. 2010Aug;28(8)868-73.

 

 

THISPRODUCTWASCITEDIN: 

 

proteome-widequantitative survey ofinvivo ubiquitylation sites reveals widespread regulatory roles. WagnerSA, etal. Molecular&CellularProteomics. 2011Sep.  

 
Proteomicanalysesrevealdivergentubiquitylationsitepatternsinmurinetissues.WagnerSA,etal. Molecular&CellularProteomics.2012Jul.

 

Systems-wideanalysisofubiquitylationdynamicsrevealsakeyroleforPAF15ubiquitylationinDNA-damagebypass.PovlsenLK,etal. NatureCellBIOLOGy. 2012Sep.

 

 

SpeciesReactivity
KeyApplicationsHostFormatAntibodyType
Human,mouse,rat,chicken,yeast,cowhorseWB,IPMousePurifiedMonoclonalAntibody
 

DESCRIPTION:Anti-Diglycyl-lysineantibody,cloneGX41
 
SPECIFICITY:Antibodyrecognizeslysineresiduesmodifiedbydiglycine,anadductleftatsitesofubiquitinationaftertrypsindigestions.
 
SOURCE/PURIFICATION:Monoclonalantibodiesareproducedbyimmunizinganimalswithasyntheticdiglycine-modifiedhistonepeptideandestablishedinhybridomalineGX41.AntibodiesarepurifiedbyproteinAandpeptideaffinitychromatography.
 
PRESENTATION:LyophilizedIgG
 
STORAGECONDITIONS:Stablefor2yearat-20ºC.
 

QUALITYASSURANCE:EachantibodybatchwillbemeasuredforitsABIlitytobindtoproteinscontainingdiglycyl-lysineandtorecoverdiglycyl-lysine-containingpeptidesfromtrypticdigestsofHEK293cells.

 

USAGESTATEMENT:Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureofantibodiesandantigens,wecannotpredictorbeheldresponsIBLewithrespecttohowLucernaTMproductswillbehaveinitscustomers"systems.ResearchersusingLucernaTMproductsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication.

 

LICENSING:CornellUniversityhasfiledausepatentonproteomicmethodsthatusethediglycyl-lysineantibodytoidentifyubiquitinatedproteins.Allcommercial usersshouldcontactCornellUniversityforauselicense.

 

GX41antibodyrecognizesthediglycyl-lysinemoietyatubiquitinatedsites.

 

 

Specificityofanti-diglycyl-lysine(cloneGX41)isverifiedbywesternblotanalysisofbeta-lactoglobulin,lysozyme,orratbrainlysate,inwhichthelysineswereeitherunmodified(A)ormodifiedwithBoc-Gly-Gly(B),respectively.

 


 

Equimolaramountsofthetwopeptides(GGDRVYIHPFHLandAc-SYSMEHFRWGK*PV-NH2)wereimmunoprecipitatedwithimmobolizedGX41antibodyandanalyzedbyMALDI-TOFMS.

 

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