OTHERTOOLSANDRESOURCES
CHEMICALSTRUCTURE
3,5-difluoro-4-hydroxybenzylideneimidazolinone-2-oxime(DHFO)
MOLECULARFORMULA:
C12H8F2N3O3
MOLECULARWEIGHT:
281.2
CASNAME/NUMBER:
(E)-4-((Z)-3,5-difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehydeoxime /
FLUORESCENCESPECTRA
A.AbsorptionandfluorescenceemissionspectraofCornTM/DFHOinpH7.4buffer. B. AbsorptionandfluorescenceemissionspectraofBroccoliTM/DFHOinpH7.4buffer.
PRESENTATION: Lyophilizeddye
QUALITYASSURANCE: Productsareanalyzedby 1HNMRandLC-MSandprovidedatpurityof>95%byHPLC.
USAGESTATEMENT: Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureoffluorophoresandaptamers,wecannotpredictorbeheldresponsIBLewithrespecttohowLucernaTM productswillbehaveinitscustomers"systems.ResearchersusingLucernaTM productsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication.
DFHOIMAGES
A. Live-cellimagingofHEK293TcellsexpressingU6promoterdrivenCornTM inthepresenceof10µMDFHO.ImagesareHoechst-stainednuclei(blue)andCornTM fluorescence(yellow),individuallyoroverlayed. ImageswereacquiredusingYFPandDAPIfiltersets. B. Live-cellimagingofE.colicellsexpressingtRNApromoterdrivenCornTM inthepresenceof10µMDFHO.
REFERENCEFORTHEPRODUCT:
SongW,FilonovGS,KimH,HirschM,LiX,MoonJD,JaffreySR.2017. ImagingRNApolymeraseIIItranscriptionusingaphotostableRNA-fluorophorecomplex. NatChemBiol.
THISPRODUCTWASCITEDIN:
LICENSING:
Lucernahastheexclusiverightstoallpatentscoveringtheuseofthisproduct. AllcommercialusersshouldcontactLucernaforauselicense.
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