产品资料

Lucerna/DFHO - 1 mg/500-1mg/1mg

 OTHERTOOLSANDRESOURCES  

 

 

 

CHEMICALSTRUCTURE

 

 

 

 3,5-difluoro-4-hydroxybenzylideneimidazolinone-2-oxime(DHFO)

 

 

MOLECULARFORMULA:

C12H8F2N3O3

 

MOLECULARWEIGHT:

281.2

 

CASNAME/NUMBER:

(E)-4-((Z)-3,5-difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehydeoxime / 

 

 

 

 

  

FLUORESCENCESPECTRA

 

A.AbsorptionandfluorescenceemissionspectraofCornTM/DFHOinpH7.4buffer.  B. AbsorptionandfluorescenceemissionspectraofBroccoliTM/DFHOinpH7.4buffer.  

 

 

PRESENTATION: Lyophilizeddye

  

 
STORAGECONDITIONS: Stableinthedarkfor2yearat-20ºC. 

 

 

QUALITYASSURANCE: Productsareanalyzedby 1HNMRandLC-MSandprovidedatpurityof>95%byHPLC. 

 

 

USAGESTATEMENT: Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureoffluorophoresandaptamers,wecannotpredictorbeheldresponsIBLewithrespecttohowLucernaTM productswillbehaveinitscustomers"systems.ResearchersusingLucernaTM productsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication. 

 

 

  

 

DFHOIMAGES 

 

 

A. Live-cellimagingofHEK293TcellsexpressingU6promoterdrivenCornTM inthepresenceof10µMDFHO.ImagesareHoechst-stainednuclei(blue)andCornTM fluorescence(yellow),individuallyoroverlayed. ImageswereacquiredusingYFPandDAPIfiltersets.  B. Live-cellimagingofE.colicellsexpressingtRNApromoterdrivenCornTM inthepresenceof10µMDFHO.

  

 

REFERENCEFORTHEPRODUCT:

 

SongW,FilonovGS,KimH,HirschM,LiX,MoonJD,JaffreySR.2017. ImagingRNApolymeraseIIItranscriptionusingaphotostableRNA-fluorophorecomplex. NatChemBiol.

 

 

 

THISPRODUCTWASCITEDIN: 

 

 

 

LICENSING: 

 

Lucernahastheexclusiverightstoallpatentscoveringtheuseofthisproduct. AllcommercialusersshouldcontactLucernaforauselicense. 

  

 

 

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