Lucerna/DFHBI 1 mg/4001 mg/1 mg

OTHERTOOLSANDRESOURCES  

 

 

 

CHEMICALSTRUCTURE

 

 

3,5-difluoro-4-hydroxybenzylideneimidazolinone(DFHBI) 

 

 

MOLECULARFORMULA:

C12H10F2N2O2

 

MOLECULARWEIGHT:

252.22

 

CASNAME/NUMBER:

4H-Imidazol-4-one,5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-,5(Z)- /1241390-29-3

 

 

  

FLUORESCENCESPECTRA

 

AbsorptionandfluorescenceemissionspectraofDFHBIinpH7.4buffer.

 

 

PRESENTATION: Lyophilizeddye

  

STORAGECONDITIONS: Stableinthedarkfor2yearat-20ºC. 

 

QUALITYASSURANCE: Productsareanalyzedby1HNMRandLC-MSandprovidedatpurityof>95%byHPLC. 

 

USAGESTATEMENT: Thisproductisintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.Duetothehighlyspecificnatureoffluorophoresandaptamers,wecannotpredictorbeheldresponsIBLewithrespecttohowLucernaTMproductswillbehaveinitscustomerssystems.ResearchersusingLucernaTMproductsshouldconductoptimizationstudiestoachievetheoptimalresultpossiblefortheirintendedapplication. 

   

 

 

DFHBIIMAGES 

 

 

HEK293Tcellsweretransfectedwitheither5S-controlaptameror5S-SpinachTM plasmidsandincubatedwith20µMDFHBI.ImagesarephaseoverlaywithHoechst-stainednuclei(blue)andSpinachTM fluorescence(green).

 

 

SpinachTM RNArobustlyactivatesthefluorescenceofDFHBI. EachtubecontainedtheindicatedsolutionandwasirrADIatedat465nm.

 

 

 

REFERENCEFORTHEPRODUCT:

 

RNAmimicsofgreenfluorescentprotein.PaigeJS,WuKY,JaffreySR.Science.2011Jul29;333(6042):642-6.

 

 

 

THISPRODUCTWASCITEDIN: 

 

RNAsignalamplifiercircuitwithintegratedfluorescenceoutput.AkterFandYokobayashiY.ACSSynthBiol.2015May15.

 

Design,synthesis,andapplicationofSpinachmolecularbeaconstriggeredbystranddisplacement.BhadraSandEllingtonAD.MethodsEnzymol.2015Jan15.

 

Live-cellimagingofmammalianRNAswithSpinach2.StrackRLandJaffreySR.MethodsEnzymol.2015Jan6.

 

MonitoringmRNAandproteinlevelsinbulkandinmodelvesicle-basedartificalcells.vanNiesP,CantonAS,Nourianz,DanelionC.MethodsEnzymol.2015Jan6.

 

UsingSpinachaptamertocorrelatemRNAandproteinlevelsinEscherichiacoli.PothoulakisGandEllisT.MethodsEnzymol.2015Jan5.

 

Invitroanalysisofriboswitch-Spinachaptamerfusionsasmetabolite-sensingfluorescentbiosensors.KellenbergerCAandHammondMC.MethodsEnzymol.2014Dec19.

 

TheSpinachRNAAptamerasaCharacterizationToolforSyntheticBIOLOGy. PothoulakisG,CeroniF,ReeveB,EllisT. ACSSynBiol.2013Sep13.

 

AsuperfoldingSpinach2revealsthedynamicnatureoftrinucleotiderepeat-containingRNA.StrackRL,DisneyMD,JaffreySR. NatMehtods.2013Dec;10(12):1219-24.

 

Genepositionmorestronglyinfluencescell-freeproteinexpressionfromoperonsthanT7transcriptionalpromoterstrength.ChizzoliniF,ForlinM,CecchiD,MansySS. ACSSynBiol.2013Dec9.   

 

 

LICENSING: 

 

Lucernahastheexclusiverightstoallpatentscoveringtheuseofthisproduct. AllcommercialusersshouldcontactLucernaforauselicense. 

 


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