Agrisera/L131 | 60S ribosomal protein L131/AS13 2650/


productinformation
Background		L13proteinislocalizedinthecytoplasm.ItbelongstotheribosomalproteinL13efamily.Alternativename:proteinBBC1homolog.
Immunogen		KLH-conjugatedsyntheticpeptidederivedfromArabidopsisthalianaUniProt:P41127,TAIR:At3g49010
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinitypurifiedserum
Format
Quantity		200µg
Reconstitution		Forreconstitutionpleaseadd100µlForreconstitution
Storage		storeat-20°C;makealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfrommaterialadheringtothecaporsidesofthetubes.
Testedapplications		westernblot(WB)
Relatedproducts		AS09478\|anti-L13-1\|60SribosomalproteinL13-1,rabbitantibodies AS08331\|anti-L-30\|50SribosomalproteinL30,rabbitantibodies AS111738\|anti-Rpl1\|50SribosomalproteinL1,rabbitantibodies AS152876 \|anti-RPL2\|ribosomalproteinL2(chloroplastic),rabbitantibodies AS142780 \|anti-RPL33\|50SribosomalproteinL33,chloroplastic,rabbitantibodies AS142781 \|anti-RPL36\|50SribosomalproteinL36,chloroplastic,rabbitantibodies AS122115 \|anti-RPL37\|ribosomalproteinL37,cytoplasmic,rabbitantibodies AS152875 \|anti-RPS1\|ribosomalproteinS1(chloroplastic),rabbitantibodies AS122114 \|anti-RPS12\|ribosomalproteinS12,chloroplastic,rabbitantibodies AS142779 \|anti-RPS15\|30SribosomalproteinS15,chloroplastic,rabbitantibodies AS10719 \|anti-Rps2\|anti-ribosomalsubunit2(cytoplasmic),rabbitantibodies AS08309 \|anti-S1\|anti-30SribosomalproteinS1,rabbitantibodies AS122111 \|anti-S14\|40SribosomalproteinS14-1,rabbitantibodies AS153068\|anti-S4\| mitochondrialribosomalsmallsubunitproteinS4,rabbitantibodies AS153067\|anti-S10\| mitochondrialribosomalsmallsubunitproteinS10,rabbitantibodies AS153069\|anti-L16 mitochondrialribosomallargesubunitproteinL16,rabbitantibodies Plantproteinextractionbuffer Secondaryantibodies
Additionalinformation

applicationinformation
Recommendeddilution		1:2500withstandardECL(WB)
Expected\|apparentMW		23.7\|29kDa(Arabidopsisthaliana)
Confirmedreactivity		Arabidopsisthaliana,Hordeumvulgare
Predictedreactivity		dicotsincluding:Brassicanapus,Nicotianatabacum,Ricinuscommunis,Vitisvinifera,monocotsincluding:Oryzasativa,Sorghumbicolor,Zeamays,trees:Piceaexcelsa,Populusbalsamifera,algae:Chlamydomonasreinhardii,
Notreactivein		diatoms
Additionalinformation		Proteinormembranesampleshouldbetreatedat70°Cfor10minbeforeloadingonthegel.
Selectedreferences		tobeaddedwhenavailable,antibodyreleasedinOctober2013.

applicationexample

10µgoftotalproteinfromArabidopsisthaliana(1)andHordeumvulgare(2)leaf,extractedwithProteinExtractionBufferPEB(AS08300),weresupplementedwith50mMDTTandheatat70°Cfor5minandkeeponicebeforeloading.ProteinseparationwasdoneusingNuPage4-12%Tris-Bisgel(Invitrogen)LDS-PAGEandblotted1htoPVDF.Blotswereblockedimmediatelyfollowingtransferin2-2.5%RPN2125(GEHealthcare)in20mMTris,137mMsodiumchloridepH7.6with0.1%(v/v)Tween-20(TBS-T)for1hatroomtemperaturewithagitation.Blotswereincubatedintheprimaryantibodyatadilutionof1:2500inblockingreagentfor1hatroomtemperaturewithagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflytwice,thenwashedoncefor15minand3timesfor5mininTBS-Tatroomtemperaturewithagitation.Blotswereincubatedinsecondaryantibody(anti-rabbitIgGhorseradishperoxidaseconjugated,recommendedsecondaryantibodyAS09602)dilutedto1:25000inTBS-Tfor1hatroomtemperaturewithagitation.Theblotswerewashedasaboveanddevelopedfor5minwithTMA-6(Lumigen)detectionreagentaccordingthemanufacturersinstructions.ImagesoftheblotswereobtainedusingaCCDimager(FluorSMax,Bio-Rad)andQuantityOnesoftware(Bio-Rad).Exposuretimewas2minutes.
Note:westernblotdetectionpatterncanbedifferentifothertypeofsamplesisused,forexamplemembranefraction.

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Agrisera/L131 | 60S ribosomal protein L131/AS13 2650/