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产品资料

lumigen/Lumi-Phos Plus/P-701/100 mL

BlottingAPSubstrate

Lumi-PhosPlusisanovel,ready-to-usechemiluminescentreagentformulationcontainingthealkalinephosphatase(AP)substrate,lumigenPPDandapatentedenhancer.Lumi-PhosPlusprovideshighlysensitivechemiluminescentdetectionofAPwithDNAprobesorantibodyconjugatesinsolutionandonsolidsupportssuchasmembranes.Thisreagentisrecommendedforeitherdirectchemiluminescentdetectionofalkalinephosphatase(AP)labelednucleicacidsorindirectlytodetecthapten-labelednucleicacidsprobedwithspecificanti-haptenantibodiesconjugatedwithAPonaSouthernblotmembraneoranyothersolidsurface.

  • Excellentsensitivity–singlecopygenedetection
  • Highintensitylightproductionwithlowbackground
  • Sustainedluminescence-withlowconcentrationsofAP,thelightintensityremainsconstantforseveralhours
  • HighstABIlityandlongshelflife

SolutionKinetics

Lumi-PhosPlushasasignificantlyhighersignaltobackgroundratiothanLumi-Phos530duetoitslowerreagentbackground.Lumi-PhosPlusreaches90%ofmaximumlightoutputapproximately27minutesafterintroductionofenzymeinhomogeneousassaysat37°C.Lightemissionismaximalat470nm.

Blotting AP Substrate

Lumi-Phos Plus

ProductSpecifications

Enzyme: 
AlkalinePhosphatase(AP)
Application: 
SouthernandNorthernBlotting,DotBlots,CDNAArrays,ColonyandPlaqueProbing
Sensitivity: 
SinglecopygenedetectionongenomicSouthernblot
StorageConditions: 
2-8°C;Storeinamberbottletoprotectfromlight
Stability: 
2yearsstoredat2-8°C
WorkingSolution: 
Readytousesolution
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