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lumigen/Lumigen ECL Extra/TM3-1000/1L

WesternBlottingHRPSubstrate

lumigenECLExtra(TMA-3)isaproprietaryacridanbasedchemiluminescentsubstrateforhighlysensitivedetectionofWesternblottedandELISAcapturedproteinsthatareboundtohorserADIshperoxidase(HRP)labeledantibodies.

  • Veryrapidonsetofchemiluminescence-imagescanberecordedinseconds
  • Excellentsensitivity-lowpicogramtomidfemtogram
  • Reducedantibodyusage-morethan10-foldreductionintheusageofexpensiveantibodyconjugates
  • Temperatureinsensitive-analyticalresultsareinsensitivetotemperaturesfrom22°C-35°Creducingtheneedforprecisetemperaturecontrol
  • Sustainedsignalduration -overseveralhoursallowsconvenientimaging

StableWorkingSolution

AsignificantfeatureofLumigenECLExtraisitsexcellentstABIlity.Unlikemostotherchemiluminescentperoxidasesubstrates,theworkingsolutionisstableatroomtemperatureforatleastoneweekandat2-8°Cforatleastonemonth.Withthisstability,theworkingsolutioncanbepreparedandstoredforlateruse.

RapidPeakIntensity

ReactionofthesubstratewithHRPenzymegeneratesrapidluminescenceofveryhighintensitywithinseconds.Peakintensityoflightemissionoccursat440nm.

ecl extra

ecl extra

ProductSpecifications

Enzyme: 
HorseradishPeroxidase(HRP)
Application: 
WesternBlottingandELISA
Sensitivity: 
Lowpicogramtomidfemtogram
StorageConditions: 
2-8°C;Storeinamberbottletoprotectfromlight
Stability: 
1yearstoredat2-8°C
WorkingSolution: 
1:1mixofAandBsolutions
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