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lumigen/Lumi-Phos 530/P-501/100 mL

ELISAAPSubstrate

Lumi-Phos530isaready-to-usechemiluminescentreagentformulationcontainingthealkalinephosphatase(AP)substrate,lumigenPPD.Lumi-Phos530isusefulforchemiluminescentdetectionofAPconjugatesinimmunoassaysandDNAprobeassays.Itprovidesultrasensitivedetectionofanalytescapturedonmagneticparticlesandinmicrotiterplatewells.Lumi-Phos530is10,000timesmoresensitivethancolorimetricsubstratesinsolution. 

  • Excellentsensitivity-lowpicogramtofemtogram
  • Sustainedluminescence-withlowconcentrationsofAP,thelightintensityremainsconstantforseveralhours
  • Linearcalibrationcurves-theluminescenceintensityatanytimepointisadirectmeasureoftheconcentrationoftheenzyme
  • HighstABIlityandlongshelflife

ChemiluminescentEnhancement

Auniqueco-surfactantsystempromotesandenhancesenergy-transfertothefluorescentheadgroupscausingthegenerationofluminescencewithabroademissionmaximumat530nm.

ELISA substrate

Lumi-Phos 530

ProductSpecifications

Enzyme: 
AlkalinePhosphatase(AP)
Application: 
Microtiterwellandbead-basedELISAassays
Sensitivity: 
Lowpicogramtofemtogram
StorageConditions: 
2-8°C;Storeinamberbottletoprotectfromlight
Stability: 
2yearsstoredat2-8°C
WorkingSolution: 
Readytousesolution
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