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Agrisera/AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (chicken)/AS03 030/188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Agrisera/AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (chicken)/AS03 030/
productinformation
Background

ATPsynthaseistheuniversalenzymethatsynthesizesATPfromADPandphosphateusingtheenergystoredinatransmembraneiongrADIent.

Immunogen

KLH-conjugatedsyntheticpeptidederivedfromavailableplant,algal(chloroplasticandmitochondrial)andbacterialsequencesofbetasubunitsofF-typeATPsynthases,includingArABIdopsisthalianachloroplasticATPsynthasesubunitbetaUniProt:P19366,TAIR:AtCg00480 andArabidopsisthalianamitochondrialATPsynthasesubunitbeta-1,UniProt:P83483, TAIR:At5g08670aswellasChlamydomonasreinhardtii,UniProt:P06541andA8IQU3

HostChicken
ClonalityPolyclonal
Clone
PurityTotalIgY
FormatLiquidat23.3µg/µlinPBSpH8.0+0.02%sodiumazideaspreservative
Quantity100µl
Reconstitution
Storage

storeat4°C;makealiquotstoavoidworkingwithastock.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromliquidmaterialadheringtothecaporsidesofthetubes.

TestedapplicationsWesternblot(WB),immunolocalization(IL)
Relatedproducts

AS05085|anti-ATPsynthasesubunitbeta,rabbitantibody

AS03030S|ATPsynthasesubunitbetaproteinstandardforquantitationandpositivecontrol

AS08304|anti-ATPsynthasesubunitalpha,rabbitantibody

AS08312|anti-ATPsynthasesubunitgamma,rabbitantibody

AS05071|anti-ATPsynthasesubunitc,rabbitantibody

collectionofantibodiestoproteinsinvolvedinbioenergeticsofaplantcell

Plantandalgalproteinextractionbuffer

Secondaryantibodies

Additionalinformation

Theanti-AtpBantibodywilldetectthemitochondrialformoftheF1ATP synthasesubcomplex,aswellasthechloroplasticCF1ATPsynthaseandmostknownbacterialF-typeATPsynthases.Peptideusedforantibodyproductionislocatedinabetasheet,whichispartlyexposednearthesurfaceoftheAtpBprotein.

applicationinformation
Recommendeddilution

1:5000-1:8000(WB),1:500forlocalizationofnativeenzymebyimmunogold(IL)

Expected|apparentMW

53.9kDa(Arabidopsisthaliana),51.7kDa(SynechocystisPCC6803),53.7kDa(Spinaciaoleracea)

ConfirmedreactivityArabidopsisthaliana,Hordeumvulgare,Spartinaalterniflora,Spinaciaoleracea,SynechocystisPCC6803,SynechococcusPCC7942,beefmuscle,ratliver
Predictedreactivity

dicotsincludingGlycinemax,Vitisvinifera andmonocotsincludingOryzasativa,Chlamydomonasreinhardtii,cyanobacteria,marinediatoms,Acinetobacterbaumannii,Clostridiumsp.,bacteriaincludingYrsiniasp.

Notreactivein

archealV-typeATPsynthase

Additionalinformation

resultsofimmunogoldstudiesusinganti-AtpBantibodyarepublishedinAnderssonetal.(2009)

SelectedreferencesQuesadaetal.(2011).ArabidopsisRUGOSA2encodesanmTERFfamilymemberrequiredformitochondrion,chloroplastandleafdevelopment.PlantJ.Nov;68(4):738-53.doi:10.1111/j.1365-313X.2011.04726.x.Epub2011Sep13.
Anderssonet.al(2009).Co-localizationofP-glyceratekinase,P-ribulokinase,ADP-glucosepyrophosphorylaseandRubiscoactivasewithCF1inpealeafchloroplasts.PlantScience177:136-14.(immunolocalization)
Morashetal.(2007).MacromoleculardynamicsofthephotosyntheticsystemoveraseasonaldevelopmentalprogressioninSpartinaalterniflora.CanadianJ.ofBotany,2007,85(5):476-483,10.1139/B07-043.

applicationinformation

western blot using anti-AtpB antibodies

10µgoftotalproteinfromsamplessuchasbeefmuscle(1),ratliver(2),Arabidopsisthalianaleaf(3),Hordeumvulgareleaf(4),SynechocystisPCC6803totalcell(5),SynechococcusPCC7942totalcell(6)wereextractedwithProteinExtractionBufferPEB(AS08300).Samplesweredilutedwith1Xsamplebuffer(NuPAGELDSsamplebuffer(Invitrogen)supplementedwith50mMDTTandheatat70Cfor5minandkeeponicebeforeloading.ProteinsampleswereseparatedonBolt4-12%Tris-Bisgel(Invitrogen)LDS-PAGEandblottedfor1hto1.5honPVDFusingBoltMiniBlotModule.Blotwasblockedimmediatelyfollowingtransferin2%blockingreagent(GERPN2125;Healthcare)dissolvedin20mMTris,137mMsodiumchloridepH7.6with0.1%(v/v)Tween-20(TBS-T)for1hatroomtemperaturewithagitation.Blotwasincubatedintheprimaryantibodyatadilutionof1:5000(inblockingreagent)for1hatroomtemperaturewithagitation.Theantibodysolutionwasdecantedandtheblotwasrinsedbrieflytwice,andthenwashed1x15minand3x5minwithTBS-Tatroomtemperaturewithagitation.Blotwasincubatedinsecondaryantibody(anti-chickenIgYhorseradishperoxidaseconjugated)dilutedto1:20000inblockingreagentfor1hatroomtemperaturewithagitation.Theblotwaswashedasabove.SignalsintheblotweredetectedusinglumigenECLUltraReagent(LumigenTMA-6,Lumigen),andvisualizedusingtheMolecularImagerVersaDocMP4000System(Bio-Rad)andQuantityOnesoftware(Bio-Rad).Exposuretimewas30seconds.


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