Agrisera/FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2/AS09 413/


productinformation
Background		FtsZ1(celldivisionproteinFtsZhomolog1)isrequiredforplastidcelldivision.Localization:pollengrainandplastidsofvegetativecells.Alternativenames:ChloroplastFtsZ,Proteinaccumulationandreplicationofchloroplasts10,Proteinplastidmovementimpaired4. FtsZ2(cellvisisionproteinFtsZhomolog2)isrequiredforplastidcelldivision.Presentintwoisoforms:FtsZ2-1andFtsZ2-2.Alternativenames:PlastiddivisionproteinFTSZ2.
Immunogen		RecombinantpartofArabidopsisthalinaFtsZconservedinFtsZ1Q42545 At5g55280andFtsZ2includingFtsZ2-1O82533,At2g36250andFtsZ2-2Q9LXJ0,At3g52750andinawiderangeofFtsZproteinsfromotherplantspecies.
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50µl
Reconstitution		Forreconstitutionadd50µlofsterilewater.
Storage		storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes.
Testedapplications		westernblot(WB)
Relatedproducts		AS07217\|FtsZ\|procaryoticcelldivisionGTPase,cyanobacterial,rabbitantibody AS10715\|anti-FtsZprocaryoticcelldivisionGTPase(bacterial),rabbitantibody AS132651\|anti-FtsZ2plantcelldivisionproteinftsZ2,rabbitantibody Plantproteinextractionbuffer Secondaryantibodies
Additionalinformation		antibodyavailablein3packsizes:50,100and150µl. Thisproductcanbesoldcontainingproclinifrequested.

applicationinformation
Recommendeddilution		1:2500withenhancedECL(WB)
Expected\|apparentMW		40kDaFtsZ1(Arabidopsisthaliana)and50kDaFtsZ2(Arabidopsisthaliana)
Confirmedreactivity		Arabidopsisthaliana,Hordeumvulgare
Predictedreactivity		Chlamydomonsreinhardtii,Cucumissativus,Gentianalutea,Glycinemax,Gossypiumarobretum,Jatrophamanihot,Liliumlongiflorum,Lupinusangustifolius,Manihotesculenta,Marchantiaaquatica,Medicagotruncatula,Morusnotabilis,Nicotianatabacum,Oryzasativa,Physcomitrellapatens,populustrichocarpa,Ricinuscommunis,Solanumlycopersicum,Sorgumbicolor,Theobromacacao,Triticumuratum,Zeamays,Yellowgentian,Vitisvinifera
Notreactivein		noconfirmedexceptionsfrompredictedreactivityknowninthemoment
Additionalinformation		tobeaddedwhenavailable
Selectedreferences		tobeaddedwhenavailable,antibodyreleasedinApril2013.

applicationexample
western blot using plant anti-FtsZ1 and FtsZ2 antibody

2µgoftotalproteinextractedwithPEB(AS08300)fromleaftissueof(1)Hordeumvulgare,(2)Arabidopsisthaliana,(3-5)recombinantFtsZ1/FtsZ2(33kDa)wereseparatedon4-12%NuPage (Invitrogen)LDS-PAGEandblotted1htoPVDF.Filterswereblocked1hwith2%low-fatmilkpowderinTBS-T(0.1%TWEEN20)andprobedwithanti-FtsZ1/FtsZ2antibody(AS09413,1:2500,1h)andsecondaryanti-rabbit(1:10000,1h)antibody(HRPconjugated,secondaryantibodyAS09602)inTBS-Tcontaining2%lowfatmilkpowder.AntibodyincubationswerefollowedbywashingsinTBS-T(15,+5,+5,+5min).AllstepswereperformedatRTwithagitation.SignalwasdetectedwithTMA-6(Lumigen)usingaFujiLAS-3000CCD(300s,standardsensitivity).Exposuretimewas2min.

新闻动态

行业前沿

技术文章

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Agrisera/FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2/AS09 413/