MatTek’s3Dtissuemodelsofthehumanairwayareadvancinginvitrorespiratoryresearchworldwide.Allowingforphysiologicalexposurestopathogens,chemicalsortherapeutics,EpiAirway’shuman-relevantBIOLOGicalresponsesarechangingthewayscientistsresearchrespiratorydiseasesanddrugdevelopment.LetEpiAirwayadvanceyourscience.
EpiAirwayisaready-to-use,3Dmucociliarytissuemodelconsistingofnormal,human-derivedtracheal/bronchialepithelialcellsalsoavailableasaco-culturesystemwithnormalhumanstromalfibroblasts(EpiAirwayFT).Culturedattheair-liquidinterface(ALI),EpiAirwayrecapitulatestheinvivophenotypesofbarrier,mucociliaryresponses,infection,toxicityresponsesanddisease.Withover100technicalreferencesavailable,EpiAirwayisamenabletoacuteorlong-termchronicstudiesacrossawiderangeofhighlypredictiveinvitroapplications.
EpiAirwayexhibitshumanrelevanttissuestructureandcellularmorphologywithhighuniformityandreproducibility.Its3DstructureconsistsoforganizedKeratin5+basalcells,mucusproducinggobletcells,functionaltightjunctionsandbeatingcilia.EpiAirwayFTincorporateshumanfibroblastsinanextracellularstromalmatrixidealforinflammationandfibrosisresearch.
TheEpiAirway3Dhumantissuemodelisroutinelyutilizedforarangeofapplicationsincludingsafety&riskassessment,anti-viralresearch,inflammation&fibrosisanddrugdelivery.Simpleprotocolsandtheevaluationofearlycellularendpointsallowscientiststoacquiredataindays,notweeksormonths.
UseEpiAirwaytostudyrespiratoryvirusattachment,replication,innateimmuneresponsesandanti-viraldrugdevelopment.
ViralInfectionReference
UtilizeexistingtransdermalpermeationequipmentorMatTek’ssimplesingleinsertpermeationdevicestoassessAPIpermeationandflux.
DrugDeliveryProtocol
DrugDeliveryReference
UseEpiAirway(availableinseveralformatsforphysiologicalexposuresystems)todeterminerelativesafetywiththeEpiAirwayIC-75assay.
EpiAirwayToxicityProtocol
InhalationToxicologyReference
EmployEpiAirway’sdiversedonorinventoryofhealthyanddiseaseddonors(Asthma,COPD,Smoker,GobletCellHyperplasia)toelucidatemolecularpathwaysandassesstherapeuticefficacy.
ViewtheMatTek ReferenceLibrary
Kit:AstandardEpiAirwaykit(AIR-100)consistsof24tissues.(Tissue“kits”containtissues,asmallamountofculturemedium,andplasticware;contactMatTekforspecifickitcontents)
Formats:
Culture:Air-liquidinterface
Histology:3-4celllayers–pseudostratified,mucociliarymorphologyandtissuestructure
Lotnumbers:Tissuelotsproducedeachweekareassignedaspecificlotnumber.Aletterofthealphabetisappendedtotheendofthelotnumbertodifferentiatebetweenindividualkitswithinagivenlotoftissues.Alltissuekitswithinalotareidenticalinregardstocells,medium,handling,cultureconditions,etc.
Shipment:At4°Conmedium-supplemented,agarosegels
Shipmentday:EveryMonday
Delivery:TuesdaymorningviaFedExpriorityservice(US).OutsideUS:Tuesday-Thursdaydependingonlocation
Shelflife:Includingtimeintransit,tissuesmaybestoredat4°Cforupto3dayspriortouse.However,extendedstorageperiodsarenotrecommendedunlessnecessary.Inaddition,thebestreproducibilitywillbeobtainediftissuesareusedconsistentlyonthesameday,e.g.Tuesdayafternoonorfollowingovernightstorageat4°C(Wednesdaymorning)
Lengthofexperiments:TissueculturescanbecontinuedforTHREE(3)MONTHSormorewithgoodretentionofnormalmorphology.Tissuesmustbefedeveryotherdaywith5.0mlofmaintenancemedium(AIR-100-MM). Cellcultureinsertsareplacedatopwashers(EPI-WSHR)orculturestands(MEL-STND)in6-wellplatestoallowusof5.0ml. Seetechnicalreference#631.
Type:Normalhumantracheal/bronchialepithelialcells(NHBE);
Geneticmake-up:Singledonor
Derivedfrom:Healthy,non-smoker(alternatedonorsavailableuponrequest)
Alternatives:NHBEfromAsthmatic,COPDandSmokerdonors
Screenedfor:HIV,Hepatitis-B,Hepatitis-C,mycoplasma
Basemedium:Dulbecco’sModifiedEagle’sMedium(DMEM)
Growthfactors/hormones:Epidermalgrowthfactorsandotherproprietaryfactors
Serum:None
Antibiotics:Gentamicin5µg/ml(10%ofnormalgentamicinlevel)
Anti-fungalagent:AmphotericinB0.25µg/ml
pHIndicator:Phenolred
Otheradditives:Proprietary
Alternatives:Phenolred-free,antibiotic-free,anti-fungal-freemediumandtissuesareavailable.Agentsareremovedatleast3dayspriortoshipment.
Assaymedium:AIR-100-ASY
Maintenancemedium:AIR-100-MM
Visualinspection:Alltissuesarevisuallyinspectedandifphysicalimperfectionsarenoted,tissuesarerejectedforshipment
End-usetesting:Transepithelialelectricalresistance(TEER)ofeachEpiAirwaylotismeasuredusinganEVOM2epithelialVolthommeterandEndohmelectrodechamber(WorldPrecisionInstruments).AminimumTEERof300Ohm•cm2isrequiredforQCrelease.
Sterility:Allmediausedthroughouttheproductionprocessischeckedforsterility.Maintenancemediumisincubatedwithoutantibioticsfor1weekandcheckedforsterility.Theagarosegelfromthe24-wellplateusedforshippingisalsoincubatedfor1weekandcheckedforanysignofcontamination
Screeningforpathogens:AllcellsarescreenedandarenegativeforHIV,hepatitisBandhepatitisCusingPCR.However,noknowntestmethodcanoffercompleteassurancethatthecellsarepathogenfree.Thus,theseproductsandallhumanderivedproductsshouldbehandledatBSL-2levels(biosafetylevel2)orhigherasrecommendedintheCDC-NIHmanual,“Biosafetyinmicrobiologicalandbiomedicallaboratories,”1998.Forfurtherassistance,pleasecontactyoursiteSafetyOfficerorMatTektechnicalservice
Notificationoflotfailure:IfatissuelotfailsourQCorsterilitytesting,thecustomerwillbenotifiedandthetissueswillbereplacedwithoutchargewhenappropriate.BecauseourQCandsterilitytestingisdonepost-shipment,notificationwillbemadeassoonaspossIBLe(Undernormalcircumstances, TEERfailureswillbenotifiedbyWednesday5p.m.;sterilityfailureswillbenotifiedwithin8daysofshipment)
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn