MatTek’s3DtissuemodelofthehumansmallintestineisourlatestinnovationadvancinginvitroGIresearchworldwide.Allowingforphysiologicalexposuresandhuman-relevantendpoints,EpiIntestinalisbeingincorporatedintopharmaceuticaldevelopmentprogramsacrosstheglobe.
TheEpiIntestinaltissuemodel(SMI-100)offersnumerousadvantagesversuscurrentlyavailablepreclinicaltestingmodelssuchasCaco-2.Thehumancell-based3Dmodelincorporatesenterocytes,panethcell,Mcells,tuftcellsandintestinalstemcellsintoahighlydifferentiated, polarizedepithelium.TheEpiIntestinalFTtissue(SMI-100-FT)includesanunderlyinglaminapropriacontainingnormalhumanintestinalfibroblastsandisusefultostudyinflammation,woundrepair,innateimmuneresponses,andfibrosis.Bothtissuesareculturedattheair-liquidinterface(ALI)toallowphysiological(luminal)exposureconditions.EpiIntestinalrecapitulates manyaspectsofnormalintestinalfunctionincluding barrier,metabolism,inflammatoryandtoxicityresponses,similartonativehumanintestinaltissue.
ImportantfeaturesofEpiIntestinalincludethepresenceoffunctionaltightjunctionsandbrushbordersattheapicaltissuesurface. Invivo,brushbordersareindirectcontactwithluminalcontentsandincludeatleast22metabolicenzymesand19drugtransporters. EpiIntestinaltightjunctionsandbrushborderscloselymimichumanstructureandphysiology.
TheEpiIntestinal3DhumantissuemodelisroutinelyutilizedforscreeningpharmaceuticalsforarangeofendpointsincludingGItoxicityandinflammation,drugmetabolism,drugpermeation,fibrosis,andintestinalinfection.Straightforwardprotocolsandtheevaluationofearlymolecularandcellularendpointsoftenallowscientiststoacquiredataindays,notweeksormonths.
UseEpiIntestinalforahighlypredictiveassaytoevaluateGItoxicityofpharmaceuticals.
GItoxicityreference
UtilizeEpiIntestinal’shighlycontrolledinvitrocultureconditionstostudyintestinalinflammation,woundrepair,andfibrosis andassesstherapeuticeffects.
IntestinalInflammationReference
UtilizeEpiIntestinalforhighlypredictiveandhuman-relevantdrugdelivery. AssessAPIbioavailABIlity(influx)andeffluxtransport,drugmetabolism,anddrug-druginteractions. EpiIntestinalcanbeco-culturedwithotherphysiologicalsystems(e.g.livercells)inmicrofluidicsystemstoexaminetheeffectofdrugmetabolitesonGItoxicityortopredictdrugpermeation/absorptionviathe“luminal”sideofthetissue.
IntestinalDrugDeliveryReference
UseEpiIntestinalFTtobetterunderstandmechanismsofwoundhealinginthegutandtoevaluatetherapeuticeffects.
IntestinalRestitutionReference
UtilizeEpiIntestinaltostudyGItractinfection,host-pathogeninteractions,andinnateimmuneresponses.
BrowseourreferencelibrarytolearnmoreabouthowresearchershaveusedourEpiIntestinaltissueintheseareasofstudy.
Kit:SMI-100andSMI-100-FTEpiIntestinalkitsconsistof24individualtissues. SMI-196orSMI-196-FTEpiIntestinalkitsconsistof96tissuesinahighthroughputscreeningplate. Eachkit containstissues,culturemediumtosupportexperimentsfor24hours,andplasticware. PleasecontactMatTekforspecifickitcontents.
Culture:Air-liquidinterface
Lotnumbers:Tissuelotsproducedeachweekareassignedaspecificlotnumber.Aletterofthealphabetisappendedtotheendofthelotnumbertodifferentiatebetweenindividualsetsof24tissueswithinagivenproductionlotoftissues.Alltissuekitswithinaproductionlotareidenticalinregardstocells,medium,handling,cultureconditions,etc.
Shipment:At4°Conmedium-supplemented,agarosegels
Shipmentday:EveryMonday
Delivery:TuesdaymorningviaFedExpriorityservice(US).OutsideUS:Tuesday-Wednesday dependingonlocation.
Shelflife:Includingtimeintransit,tissuesmaybestoredat4°Cforupto2dayspriortouse.However,thebestreproducibilitywillbeobtainediftissuesareusedconsistentlyonthesameday,e.g.Tuesdayafternoonorfollowingovernightstorageat4°C(Wednesdaymorning).
Lengthofexperiments:TissueculturescanbecontinuedforONE(1)MONTHormorewithgoodretentionofnormalmorphology.Tissuesmustbefedeveryotherdaywith5.0mlofmaintenancemedium(SMI-100-MM).Cellcultureinsertsareplacedatopwashers(EPI-WSHR)orculturestands(MEL-STND)in6-wellplatestoallowuseof5.0ml.
Type:Primaryhumansmallintestinalepithelialcells(HSIE)
Geneticmake-up:Singledonor
Derivedfrom:Healthyadultdonor
Alternatives:Alternatedonorsavailableuponrequest
Screenedfor:HIV,Hepatitis-B,Hepatitis-C,mycoplasma
Basemedium:Dulbecco’sModifiedEagle’sMedium(DMEM)
Growthfactors/hormones:Epidermalgrowthfactorandotherproprietaryfactors
Antibiotics:Gentamicin5µg/ml
Anti-fungalagent:AmphotericinB0.25µg/ml
pHIndicator:Phenolred
Otheradditives:Proprietary
Alternatives:Phenolred-free,antibiotic-free,anti-fungal-freemediumandtissuesareavailable.Agentsareremovedatleast3dayspriortoshipment.
Visualinspection:Alltissuesarevisuallyinspectedandifphysicalimperfectionsarenoted,tissuesarerejectedforshipment
Sterility:Allmediausedthroughouttheproductionprocessischeckedforsterility.Maintenancemediumisincubatedwithandwithoutantibioticsfor1weekandcheckedforsterility.Theagarosegelfromthe24-wellplateusedforshippingisalsoincubatedfor1weekandcheckedforanysignofcontamination
Screeningforpathogens:AllcellsarescreenedandarenegativeforHIV,hepatitisBandhepatitisCusingPCR.However,noknowntestmethodcanoffercompleteassurancethatthecellsarepathogenfree.Thus,theseproductsandallhumanderivedproductsshouldbehandledatBSL-2levels(biosafetylevel2)orhigherasrecommendedintheCDC-NIHmanual,“BiosafetyinmicroBIOLOGicalandbiomedicallaboratories,”1998.Forfurtherassistance,pleasecontactyoursiteSafetyOfficerorMatTektechnicalservice
Notificationoflotfailure:IfatissuelotfailsQCorsterilitytesting,thecustomerwillbenotifiedandthetissueswillbereplacedwithoutcharge.BecauseourQCandsterilitytestingisdonepost-shipment,notificationwillbemadeassoonaspossIBLe(Undernormalcircumstances,ET-50failureswillbenotifiedbyThursday5p.m.;sterilityfailureswillbenotifiedwithin8daysofshipment)
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn