MCLAB/BigDye Terminator 5X Sequencing Buffer/SBUFOEM/1 Ea

Description:
TheBigDye^®Terminator5XSequencingBufferreducessequencingcostswithoutaffectingsequenceaccuracyorreadlength.Itsbuffersystemenablestouselessdyeterminatormix.

Applications:
DNAsequencing
Fragmentanalysis

RecommendedStorageCondition: 4°C

RecommendedProtocol:
Preparesequencingreactions(10µlrxn)accordingtothefollowing:
-Adddyeterminatormix0.25-0.5µl
-Add5XSequencingBuffer2µl
-Addtemplate(100-200ng/µl)1µl
-Addprimer(3pmol/µl)1µl
-AddH₂Oto10µl
Cyclethereactionaccordingtothefollowingprotocol:

Step1:96°C 3minutes
Step2:96°C 10seconds
Step3:50°C 5seconds
Step4:60°C 2minutesand30seconds
(25to30cyclesfromstep2tostep4)
Step5:4°Chold

Notes:
Othercycleconditionsmayworkwellforindividualusers.Theseconditionsworkwellformostofthesamplesprocessedinourlabusingreducedvolumeandreducedterminatormixconcentrations.