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MCLAB/2X OriMaster Mix/OMM100/1 Ea

The2XOri-MasterMixisapremixed,ready-to-usesolutioncontainingahigh-fidelityDNApolymerase,orispecificprimers,dNTPs,andMgCl2.

Description:
The2XOri-MasterMixisapremixed,ready-to-usesolutioncontainingahigh-fidelityDNApolymerase,orispecificprimers,dNTPs,andMgCl2.TheMasterMixcanproducelinearcopiesofbacterialplasmidsfromasinglecolony.Thereactionmixtureandconditionsareoptimizedregardlessoftheplasmidcopynumber.Amplifiedproductscanbedirectlysequencedwithouttheneedforpurification.

Applications:

  • ColonyPCR
  • DNAsequencing
  • RestrictionDigestion

Features:

  • Fastreaction:lessthan2hrsfromacolonytoitsDNAproducts,comparingto16-24hoursforaminiprepprocess
  • Goodyield:a25μlreactionyieldsupto1μgDNA
  • Easytouse:Ori-specificprimersareincludedandthePCRprotocolisoptimized.Justaddthetemplateandwater.
  • Longamplificationfragments:upto10Kb
  • Highthroughputcapability:96or384wellplatecompatible

ExperimentalData:




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