MCLAB/T7 DNA Ligase/T7DL100/1,500 Weiss units

Description:
T7DNALigasecatalyzestheformationofaphosphodiesterbondbetweena5'-phosphateanda3'-hydroxylterminiinduplexDNA.Theenzymewilljoinbluntendsandcohesiveendsterminiaswellasrepairsingle-strandednicksinduplexDNA.

Application:
-JoiningofOkazakifragmentsduringreplication
-Completingshort-patchDNAsynthesisoccurringinDNArepairprocess

Source:
ArecombinantE.colistraincarryingtheT7DNALigasegene.

Suppliedin:
20mMTris-HCl
300mMNaCl
1mMDTT
0.1mMEDTA
50%Glycerol
pH7.5@25°C

10xRapidLigationBuffer:
660mMTris-HCl
100mMMgCl₂
10mMDTT
10mMATP
75%PEG6000
pH7.6@25°C

UnitDefinition:
OneWeissunitoftheenzymecatalyzestheconversionof1nmolof[³²PP_i]intoNorit-adsorbableformin20minat37°C. Enzymeactivityisassayedinthefollowingmixture:66mMTris-HCl(pH7.6),6.6mMMgCl₂,0.066mMATP,10mMDTT,3.3µM[³²PP_i].

OneWeissUnitisapproximatelyequivalentto67cohesiveendunits(CEU).Or1000CEU=15WeissUnit.

SpecificActivity: 45WeissU/µg

RecommendedStorageCondition:-20°C

Reference:
Doherty,A.etal.J.Biol.Chem(1996)V.271,No.19,11083-11089.