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| Description: MCLAB'sRNA-SeqLibraryConstructionKitisahighlyefficientlibraryconstructionkitforpreparingsingle,paired-endormultiplexedcDNAlibrariesforhigh-throughputsequencing.ThiskitcanbeusedtoconvertRNAtranscriptsintocDNAwholetranscriptomelibrariesorsmallRNAlibrariesfornext-generationsequencinganalysisusingIllumina®GAIIxTM,HiSeqTM2000andMiSeq®sequencingplatforms.Withpreservingthecomplexityofsequencinglibraries,theprotocolisquickerthanthestandardmethodandcouldbeusedroutinelyforRNAsequencingthroughtheIllumina®platform. Function: ThesuperiorfunctionofMCLAB'sRNA-SeqLibraryConstructionKitdependsonourproprietaryenzymesystems.Uniqueimprovementtoeachkeyenzymeatspecificstepsinthelibraryconstructionworkflowincreasessensitivity,flexibilityandspeedfornext-generationsequencing.ThetargetRNAfragmentscanbetaggedwithlinkersatbothendsbyRNAligases(Cat#T4RL1-100andT4RL2T-100).CorrespondingcDNAcanbesynthesizedbyUniversalReverseTranscriptase(Cat#SSII-100).ThecDNAlibrarycanbeenrichedbyPCRwithhighfidelityPfuDNAPolymerase(Cat#AD-200).Aftergel-orbeads-basedpurificationforsizeselection,finalcDNAlibrariescanhybridizedirectlytotheoligonucleotidesontheflowcellsurfaceforclustergenerationandsequencingthereafter. Features: -Simplifiedworkflow,reducinghands-ontime -Optimizedreactionconditions,increasingsensitivity -Straightforwardprotocolminimizingpriorexperienceneeded -Strand-specificsequencingdata -Cost-effectivewithallkeyenzymes -Highflexibility:multiplexsamplesandautomationfriendly -FunctionallyvalidatedwithIllumina®GAIIxTM,HiSeqTM2000andMiSeq®sequencingplatforms RecommendedStorageConditions:-20°C. |