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MCLAB/DNA Polymerase I/DPI200/10,000 units
DNAPolymeraseIisa DNA-dependentDNApolymerase.Theenzymecatalyzes5'->3'synthesisofDNAandexhibits3'->5’exonucleaseproofreADIngactivity.

Description:
DNAPolymeraseIisamesophilicDNApolymerasethatexhibits5'->3'DNAsynthesisactivityinadditiontoboth3'->5'and5'->3'exonucleaseactivities.ThecombinationofDNAsynthesisand5'->3'nucleasecharacteristicsenablenick-translationduringDNAsynthesis.

Application:
-NicktranslationofDNAtoobtainprobeswithahighspecificactivity
-SecondstrandsynthesisofCDNA

Source:
ArecombinantE.colistraincarryingthePolAgene.

SpecificActivity:6,850U/mg

Suppliedin:
25mMTris-HCl
0.1mMEDTA
1.0mMDTTl
50%Glycerol
pH7.4@25°C

Suppliedwith:
10xBlueBuffer

10xBlueBuffer:
500mMNaCl
100mMTris-HCl
100mMMgCl2
10mMDTT
pH7.9@25°C

UnitDefinition:
1unitisdefinedastheamountofpolymeraserequiredtoconvert10nmolofdNTPsintoacidinsolublematerialin30minutesat37°C.

RecommendedStorageCondition:-20°C


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