MCLAB/HB101 Competent E. Coli/HB100/1 Ea

Description:
HighstABIlity,highefficiency,noheatshockstep.ForResearchUseOnly.

Application:

• Idealforsub-cloningandscale-upapplications
• HB101strainisahybridK12xBbacterium,containingtherecA13mutationthatminimizesrecombinationandaidsininsertstability.Inaddition,itcarriesthehsdS20(rB-mB-)restrictionminusgenotypewhichpreventscleavageofclonedDNAbyendogenousrestrictionenzymes.HB101straindoesnotsupportAlpha-complementationforblue/whitescreening.Transformefficiencyisaround1.0x10⁷~1.0x10⁹cfu/µgwithpUC18controlDNA.

Recommendedstoragecondition:
Thisproductshouldbestoredat-80°C.Thawoniceonlybeforeuse.Donotre-freeze.

Recommendedreactionconditions:

• MixDNAwithcompetentcells
• Letstandonbenchfor5minutes
• Directplate

Genotype:
F-mcrBmrrhsdS20(rB-mB-)recA13leuB6ara-14proA2lacY1galK2xyl-5mtl-1rpsL20(SmR)glnV44λ-

Reference

1. Boyer,H.W.,Roulland-Dussoix,D.J.Mol.Biol.,(1969)41:459-472

CompetentCellType:
Chemically

TransformationEfficiency(cfu/μg):
>1x10⁹

Blue-WhietScreening:
No

Strain:
Hybrid

ReducesRecombination:
Yes

CloningMethylatedDNA:
Yes

ImprovesPlasmidQuality:
Yes

PreparingUnmethylatedDNA:
Yes

T1PhageResistant:
No

RecADeficient:
Yes