Description:
ExtremeThermostableSSBisasingle-strandedDNAbindingproteinisolatedfromahyperthermophilicmicroorganism.Itremainsfullyactiveafterincubationat95°Cforupto60minutes.DuetoitsABIlitytowithstandextremelyhightemperatureenvironments,ETSSBcanbeusedinapplicationsthatrequireextremelyhightemperatureconditions,suchasnucleicacidamplificationandsequencing.
Source:
PurifiedfromanE.colistrainthatoverexpressestheSSBgeneisolatedfromahyperthermophilicmicroorganism.
Application:
-ImprovestheyieldofmultiplexPCRandmultiplexHAD
-IncreasestheyieldandprocessivityofRTduringRT-PCR(1,2)
-IncreasestheyieldandspecificityofPCRreactions(3-7)
-ImprovestheprocessivityofDNApolymerase(8)
-StabilizationandmarkingofssDNAstructure(9)
-ImprovesDNAsequencingthroughregionswithstrongsecondarystructure(6)
-EnhancestheRecAactivityforssDNAbindingandstrandtransfer(10,11)
UnitDefinition:
SoldbymassofpureproteinasdeterminedbyOD280.
RecommendedStorageCondition:-20ºC
Reference:
1.Baugh,L.R.,Hill,A.A.,Brown,E.L.&Hunter,C.P.(2001)NucleicAcidsRes29,E29.
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3.Schwarz,K.,Hansen-Hagge,T.&Bartram,C.(1990)NucleicAcidsRes18,1079.
4.Chou,Q.(1992)NucleicAcidsRes20,4371.
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6.Rapley,R.(1994)MolBiotechnol2,295-8.
7.Olszewski,M.,Rebala,K.,Szczerkowska,Z.&Kur,J.(2005)MolCellProbes19,203-5.
8.Myers,T.W.&Romano,L.J.(1988)JBiolChem263,17006-15.
9.Delius,H.,Mantell,N.J.&Alberts,B.(1972)JMolBiol67,341-50.
10.Reddy,M.S.,Vaze,M.B.,Madhusudan,K.&Muniyappa,K.(2000)Biochemistry39,14250-62.
11.West,S.C.,Cassuto,E.&Howard-Flanders,P.(1982)MolGenGenet186,333-8. |
