MCLAB/PstI/PSTOEM/Any Size

Recognitionsite:CTGCA^G

Source:
PurifiedfromastrainofE.colithatbearstheclonedPstIgene.

StorageBuffer:
10mMTris-HCl(pH7.4),50mMNaCl,0.1mMEDTA,1mMDTT,50%(v/v)TritonX-100.

10xPstIBuffer
100mMNaCl
50mMTris-HCl
10mMMgCl₂
100μg/mlBSA
pH7.9@25°C

ReactionCondition:
Bufferat37°C.

TypicalLigation/RecutAssayResults:
PstI-cleavedλDNAis≥95%ligatedafter1h.Oftheligatedfragments,100%arecleaved(recut)byPstI.

RecommendedStorageCondition:-20°C.

Comments:
DoesnotcleaveDNAwheneithertheAorthe5’CresidueisN6–methyladenineor5-methylcytosine,respectively.Partiallyresistanttoheatinactivation(10minutes,65°C).Glycerolconcentrations>5%(v/v)mayalterspecificity.